层粘连蛋白在甲状腺乳头状癌组织的表达及其意义

Expression and its signification of laminin in thyroid papillary carcinomas

目的:探讨层粘连蛋白(laminin,Ln)与甲状腺乳头状癌的侵袭和淋巴结转移的相关性。方法:应用免疫组织化学方法检测20例腺瘤、64例甲状腺乳头状癌的Ln蛋白表达情况。结果:Ln的表达在甲状腺乳头状癌的阳性率为82.8%,腺瘤的阳性率为40.0%,差异有显著性(P<0.01)。Ln的表达Ⅰ级、Ⅱ级、Ⅲ级的病人中有淋巴结转移者分别占16.7%、58.1%90.0%,差异有显著性(P<0.005)。Ln在胞质表达的病人中有淋巴结转移者21.2%,无淋巴结转移者3.2%,差异无显著性(P>0.05)。结论:在甲状腺乳头状癌中,Ln与良恶性、淋巴结转移特性有相关性;发现在淋巴结转移和浸润前缘的瘤细胞出现胞质表达及弥散分布和渗透现象。

Objective： To investigate the relationship between laminin and invasion, lymph node metastasis in thyroid papillary carcinoma.Methods： Laminin was detected by immounohistochemistry S-P method in 20 thyroid adenoma cases and 64 thyroid papillary carcinoma cases. Results：The positive rate of laminin in the thyroid papillary carcinoma patients was 82.8 % and 40.0 96 in adenoma patients, the difference was significant （ P 〈 0.01）. The rates of lymph node metastasis were 16.7 %, 58.1% and 90.0 % respectively in the laminin expression of grade from Ⅰ to Ⅲ, the difference was significant （P 〈 0. 005）. The rate of laminin expression in cytoplasm was 21.2 % in patients with lymph node metastasis, but 3.2 % in patients without lymph node metastasis. The difference was not significant（P 〉0.05）. Conclusions：In the thyroid papillary carcinoma, the expression of laminin is related to benign,malignant and metastasic tumors. The phenomena of dispersion, penetration and cytoplasmic expression are found in the neoplastic cells invading brim and metastasic lymph node.