351株葡萄球菌的鉴定及药敏试验

Identification and drug sensitivity test of 351 staphylococcus strains

目的:了解葡萄球菌的临床感染情况及耐药性,为合理用药提供科学依据。方法:对各科住院病人的标本中分离出来的351株葡萄球菌进行菌株鉴定、耐甲氧西林葡萄球菌(MRS)筛选、β―内酰胺酶定性试验及药敏试验。结果:葡萄球菌引起的感染中以呼吸系统最常见,占65.0%(228/351);MRS的分离率较高为85.2﹪(299/351),其中耐甲氧西林金黄色葡萄球菌(MR-SA)为81.0﹪(145/179);β-内酰胺酶定性试验阳性率高达91.5%(321/351);除万古霉素外,351株葡萄球菌对8种抗菌药物的耐药率为68.9%～97.7%。结论:耐甲氧西林葡萄球菌是目前医院内感染的重要病原菌之一,且感染率有上升趋势。

Objective： To investigate the prevalence and the drug sensitivity of staphylococcus in clinic so as to provide scientific basis for rational drug use. Methods： 351 staphylococcus strains isolated from inpatient specimens were identified, and screening test for methieillin-resistant staphylococcus （MRS）, qualitative determination for β -laetamase and testing for bacterial sensitivity to antimierobial agents were performed. Resdts： Staphyloeoeeus were dramatically found in respiratoty infection, accounting for 65.0% （228/351）. The isolation rate of MRS was 85.2% （29W351） and MRSA 81.0% （145/179）. Staphylococcus strains with positive β-laetarnase were 91.5%（321/351）. The resistance rates of staphylococcus to 8 antibiotics were 68.9% to 97.7%, except vaneomyein. Conclusion： MRS is one of the important pathogens causing nosoeomial infection and its infection rate is trending upward.