视神经鞘切开减压术对大鼠视神经挤压伤后RGC凋亡的影响

Optic nerve sheath incision decompression on retinal ganglion cell apoptosis after optic nerve crush of rat

目地 观察早期视神经鞘切开减压术对大鼠视神经挤压伤后RGC凋亡的相关机制。方法 大鼠91只分为对照组、损伤组、手术组各7、42、42只通过视网膜切片技术，HE染色，免疫组化SP法于伤后3、7、15各时间点视网膜神经节细胞计数及检测BCL-2和BAX阳性细胞数。结果 手术组各时间点RGC计数均高于损伤组，差异有显著性(P＜0.05)。手术组各时间点BCL-2阳性细胞数均高于损伤组，BAX阳性细胞数均低于损伤组，差异有显著性(P＜0.05)。结论 早期视神经鞘切开减压术可对大鼠视神经挤压伤后能上调BCL-2基因的表达和下调BAX基因的表达而抑制RGC的凋亡。

Objective To investigate the effect of optic nerve sheath incision decompression （ONSD） on optic nerve incomplete injuries . Method ninety -one SD rats were divided into normal control crash control and surgical groups. The retinal ganglion ceils ,BCL -2 and BAX positive cells were calculated at day 3 ,7 15 after crush respectively. Results the number of BAX positive cells in surgical group were less then those in crush group significantly （ P 〈 0. 05）. , while the number of retinal ganglion and BCL - 2 positive cells in surgical groups were more than those in crush group at day 3,7,15 after crash significantly （ P 〈 0.05 ）. Conclusion Optic nerve sheath incision decompression （ONSD） can improve the survival of RGC through up - regulation of BCL - 2 gene expression and down - regulation of BAX gene expression after optic nerve crush.