肺缺血再灌注损伤的超微结构改变及红花对其的影响

Effects of safflor injection on the ultrastructure changes in lung tissue during the ischemia-reperfusion lung injury in rabbits

目的：观察红花注射液对肺缺血再灌注损伤超微结构的影响，探讨其机制。方法：复制在体免 肺缺血再灌注损伤模型。30只日本大耳免，随机均分为三组：假手术组(S 组)，缺血再灌注组(IR 组)和 缺血再灌注+红花注射液组(ST 组)。使用电子显微镜观察各组肺组织标本超微结构的变化，作对比分 析。结果：IR 组主要异常有肺毛细血管内皮细胞肿胀、连续性中断，线粒体水肿变性，管腔内大量中性 拉细胞附壁，部分堵塞管腔；Ⅱ型肺泡上皮细胞微绒毛减少，线粒体肿胀，板层小体稀少；肺间质和肺 泡水肿。SI 组在上述部位的病理变化均明显减轻。结论：红花可减轻肺缺血再灌注时超微结构异常改变， 其机制是遏制激活的中性粒细胞和功能失调的血管内皮细胞的相互作用所启动的一系列微血管和肺组织 细胞的损伤。

Objective： To observe ultrastructure changes of safflor injection in protecting lung against ischemia-reperfusion indury and to study the mechanism. Methods： Rabbit lung model of ischemia-reperfusion injury was constituted in vivo. Thirty rabbits were divided into three groups randomly： sham-operation group（group S）, ischemia-reperfusion group（group IR） and ischemiareperfusion plus safflor injection（group SI）, the ultrastructure changes in lung tissue were observed under electron microscope at 3 hours after reperfusion. Results： In IR group, it could be seen that swollen vascular endothelial cells with gathered chromatin along the nuclear membrane, reduced microvilli on the surface of type ~ alveoli with swollen mitochondria, and inflammation cells infiltration in pulmonary septa. The basement membrane was disrupted, safflor injection treatment mitigated the injury of IR in SI group. Conclusion： The ultrastructure of pulmonary alveoli and its capillaries were certainly changed in a state of ischemia-reperfused lung, and Safflor can decrease the injury by inhibition of interactions between infiltration of polymorphonuclear neutrophils and functional disordered vascular endothelial.