用于晶体衍射和核磁共振研究的蛋白质自动化生产

Protein automatic production for crystallography and NMR spectroscopy

介绍了用于晶体衍射研究和NMR研究的蛋白质自动化生产的步骤,这些步骤包括建立克隆和表达载体,蛋白质的表达,蛋白质纯化,蛋白质质量控制和NMR或晶体衍射方法的选择。

This paper introduces the steps of protein automatic production for crystallography and NMR spectroscopy which consist of establishment of clone and expression constructs, protein expression, protein purification, quality control of purified proteins, method selection of NMR or crystallography.