摘要
This study aims to produce an effective subunit vaccine against infectious bursal disease virus （IBDV）. The genes of chicken interleukin-2 （ChIL-2） and IBDV viral protein 2 （VP2） were amplified and fused by splice overlap extension-polymerase chain reaction （SOE-PCR）. The fusion gene was digested by EcoR I/Kpn I and inserted into pBacPAK8 vector, resulting in recombinant transfer plasmid pBacPakVP2-IL2. The recombinant plasmid was transfected into Sf-9 cells accompanied with hybrid nuclear polyhedrosis virus （HyNPV） genome DNA and lipofectin. Plaque-purification indicated that we had got the recombinant Hy-VP2-IL2. Fusion protein VP2-IL2 was expressed effectively both in insect cells and bombyx mori. The expression of fusion protein was confirmed by ELISA, SDS-PAGE and Western blotting assay, respectively. This efficient system allows us to meet the need for inexpensive vaccines required by the poultry industry. Cellular ＆ Molecular Immunology. 2005;2（3）：231-235.
This study aims to produce an effective subunit vaccine against infectious bursal disease virus （IBDV）. The genes of chicken interleukin-2 （ChIL-2） and IBDV viral protein 2 （VP2） were amplified and fused by splice overlap extension-polymerase chain reaction （SOE-PCR）. The fusion gene was digested by EcoR I/Kpn I and inserted into pBacPAK8 vector, resulting in recombinant transfer plasmid pBacPakVP2-IL2. The recombinant plasmid was transfected into Sf-9 cells accompanied with hybrid nuclear polyhedrosis virus （HyNPV） genome DNA and lipofectin. Plaque-purification indicated that we had got the recombinant Hy-VP2-IL2. Fusion protein VP2-IL2 was expressed effectively both in insect cells and bombyx mori. The expression of fusion protein was confirmed by ELISA, SDS-PAGE and Western blotting assay, respectively. This efficient system allows us to meet the need for inexpensive vaccines required by the poultry industry. Cellular ＆ Molecular Immunology. 2005;2（3）：231-235.
基金
This work was supported in part by the grants from the National High Technology Research and Development Program of China(No.2004BA757C)
the Key Project of Zhejiang Province(NO.011102465).
