影响苏云金芽孢杆菌基因在转基因植物中表达的因素

Factors Affecting Expression of Bacillus thuringiensis Genes in Transgenic Plants

苏云金芽孢杆菌(Bacillusthuringiensis,Bt)杀虫晶体蛋白基因是植物抗虫基因工程中应用最广泛的基因资源。影响Bt基因在转基因植物中表达的因素繁多,阐明这些因素的效应对于获得Bt基因在受体植物中的稳定高效表达具有重要意义。现对Bt基因表达的主要影响因子,如Bt基因表达单元、植物发育、外部环境条件、受体植物遗传背景、整合位点及Bt基因沉默现象等进行了综述。

The insecticidal crystal protein gene from Bacillus thuringiensis （Bt） is the most widely used resource in plant engineering for insect resistance to date. There are many factors affecting Bt gene expression, elucidating their effects is important to acquire stable and high expression of Bt gene in transgenic plants. Effects of main factors on Bt gene expression, such as the expression cassette of Bt gene, plant development, external environmental conditions, genetic background of receptors and integration site in plant genome, and Bt gene silence were reviewed in the paper.