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Ames实验及彗星实验检测辅酶NADH的抗突变作用 被引量：2

The Anti-mutagenicity of Reduced Coenzyme NADH by Ames Assay and Comet Assay
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摘要 采用Ames实验及单细胞凝胶电泳(SCGE,彗星实验),对还原型辅酶NADH进行抗突变研究。NADH中、高剂量组在加或不加S9的情况下,均能不同程度地抑制由致突变物引起的TA98、TA100回变菌落数的增加,降低SCGE拖尾细胞率。表明还原型辅酶NADH具有一定的抗突变作用。 The anti-mutagenicity of reduced coenzyme NADH was studied by Ames assay and single cell gel electrophoresis assay （SCGE, comet assay）. The results showed that the the increasing of the frequency of revertant colonies caused by treating TA98, TA100 with positive mutagens could be inhibited by the middle and high dose of reduced coenzyme NADH, with or without S9. The comet assay showed that the length of DNA migration （the length of the tail of comet cells） and the percentage of migrated DNA were decreased by the middle and high dose of NADH. It indicates that the reduced coenzyme NADH might be able to inhibit mutagenesis caused by mutagens.
作者 温居一 孙海 张积仁
机构地区 南方医科大学珠江医院肿瘤中心
出处 《细胞生物学杂志》 CSCD 2005年第5期593-596,共4页 Chinese Journal of Cell Biology
基金 全军医学科研"十五"计划基金资助项目(No.01MA138)~~
关键词 AMES实验 彗星实验 辅酶NADH 抗突变作用 药物不良反应 化疗药物 细胞毒药物 reduced coenzyme NADH anti-mutagenicity Ames assay comet assay
分类号 Q552 [生物学—生物化学]
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细胞生物学杂志
2005年 第5期

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