利用组织芯片技术研究PTEN和VEGF在胃癌中的表达及意义

Expression and clinical significance of PTEN and VEGF proteins in human gastric carcinoma detected using tissue microarray

目的研究PTEN和血管内皮生长因子(vascularendothelialgrowthfactor,VEGF)在胃癌中的表达及临床意义。方法应用组织微阵列仪制作97孔胃癌组织芯片(tissuemicroarray)。用免疫组织化学S-P法检测PTEN、VEGF在72例胃癌和25例正常胃黏膜中的表达。结果胃癌组织中PTEN蛋白阳性表达率显著低于正常胃黏膜(45.8%vs100%,P<0.01);VEGF的阳性表达率显著高于正常胃黏膜(75%vs12%,P<0.01),PTEN在胃癌中的表达与VEGF呈负相关(P<0.01)。PTEN、VEGF的表达在中高分化腺癌分别为68.8%、62.5%(P>0.05),在低分化及未分化腺癌分别为27.5%、85.0%(P<0.05);伴淋巴结转移者分别为31.6%、86.9%(P<0.05),无淋巴结转移者分别为61.8%、61.8%(P>0.05);临床病理分期Ⅰ+Ⅱ期分别为57.1%、61.9%(P>0.05),Ⅲ+Ⅳ期分别为30.0%、93.3%(P<0.05);与性别、年龄、肿瘤大小和组织分型无显著差异(P>0.05)。结论PTEN失活或蛋白表达降低、VEGF的高表达与胃癌临床病理特征和生物学行为有密切关系,PTEN在低分化或未分化以及伴淋巴结转移和临床Ⅲ+Ⅳ期胃癌中的表达与VEGF呈负相关。联合检测PTEN、VEGF对胃癌的恶性程度及预后判断具有一定的临床参考意义。应用组织芯片大规模高效检测临床组织样本是可行的,具有快速、准确、方便经济的特点。

Objective To investigate the expressions and clinical significance of PTEN and vascular endothelial growth factor （VEGF） proteins in human gastric carcinoma and normal gastric mucosa. Methods Tissue microarray was used in 72 cases of gastric carcinoma and 25 cases of normal gastric mucosa. The expressions of PTEN and VEGF proteins were detected using immunohistochemical S-P method. Results In gastric carcinoma, the positive expression rates of PTEN and VEGF proteins were 45.8%（33/72） and 75%（54/ 72）, while 100%（25/25） and 12%（3/25） in normal mucosa, respectively, showing the expressions of PTEN and VEGF proteins in cancer tissues were significant difference when compared with those in normal mucosa （P 〈 0.01, P 〈 0.01）. The expressions of PTEN and VEGF proteins in the well-differentiated and mid-differentiated carcinoma groups were 68.8% and 62.5%; those in poorly differentiated and undifferentiated carcinoma groups were 27.5% and 85.0%, respectively. The expressions of PTEN and VEGF proteins were significantly corre, lated with infiltrating depth, cell differentiation, lymph node metastasis and clinical stages, but not with gender, ages, tumor volume and histological classification. The expression of PTEN was negatively related to the expression of VEGF in gastric carcinoma （P 〈 0.01）. Conclusion PTEN and VEGF proteins are significantly correlated with the clinicopathological characteristics and biologic behaviors in gastric carcinoma. Combined detection of PTEN and VEGF might be helpful to evaluate malignant degree and prognosis of gastric carcinoma. It is believed that tissue microarray is a rapid, economic and accurate tool in screening the clinical tissue specimens on a large scale.