Rb基因在小鼠胚胎发育中期肾组织的表达

The Expression of Rb Gene in the Developing Kidney of Embryo Mouse

用原位杂交法检测小鼠肾组织中细胞周期调控基因Rb的表达;用免疫组织化学及缺口末端标记法观察小鼠肾组织发育过程中的细胞增殖与凋亡。结果表明,Rb基因主要在肾上皮细胞表达,随胎龄增长而表达颜色加深。肾组织在第13天有一个较大的增殖增长,到第14天生长趋于稳定。以上结果说明Rb基因在小鼠胚胎肾发育中期有表达,该基因在胚胎肾发育中期起一定的作用;同时胚胎发育中期肾细胞增殖与凋亡相伴存在。

The expression of Rb gene and the dynamic trends of cell proliferation, apoptosis in developing kidney of mouse were studied. The kidney tissues were taken from different developmental phases of Kunming mouse. The expression of Rb gene was detected by in situ hybridization. Immunohistochemistry and TUNEL techniques were adopted to observe the variation of cell proliferation and apoptosis. With the development of embryo, the expression of Rb have a darker colour in the epidermic cell of kidney. The cell proliferation increased greatly in the 13th day. The possible function of Rb might be correlated to the developmental process of middle embryo kidney. Cell proliferation was accompanied by apoptosis during the development of mouse kidney. The dynamic changes of both ratios might be correlated to the different structures appearing in different developmental process.