一种快速提取植物病毒DNA的方法

A Rapid Plant DNA Viruses Release Protocol from Plant Tissue

介绍在PCR检测体系中一种快速提取病毒DNA的方法。利用高盐缓冲液溶液释放病毒DNA,同时利用葡聚糖凝胶微柱纯化提取液,有效消除样品中PCR抑制物质并直接作为PCR反应模板扩增检测病毒。该法无需任何特殊设备,适合对大量植株进行大通量的检测分析。

A rapid plant tissue preparation protocol suitable for the detection of DNA viruses by the polymerase chain reaction （PCR） was described. Liberation of viruses from leaves in an appropriate buffer and high salt may allow sufficient template release for direct use in PCR. Single-use Sephadex gel columns provide excellent purification of crude sap which can reduce plant inhibitory factors which may interfer with PCR and introduced the extracted sap directly into PCR reaction mixture.