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转基因动物乳腺生物反应器的发展概况及人工染色体在其中的应用 被引量：1

Advancees in Production of Transgenic Animals as Mammary Gland Bioreactors and the Use of Artificial Chromosomes
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摘要 转基因动物乳腺生物反应器位点效应的影响是制备转基因动物乳腺生物反应器过程中的主要问题。酵母人工染色体(YAC)和细菌人工染色体(BAC)具有容量大的特性,可以将乳蛋白的整个基因座包括所有调控序列全部装载进去,有可能克服位点效应的影响,是一种理想的载体。YAC和BAC载体转基因技术可能成为避开基因打靶获得高效表达的转基因动物乳腺生物反应器的另一途径。 The position effect is one major problem in the production of transgenic animals as mammary gland bioreactors. Given the high capacity of yeast artificial chromosomes （YAC） and bacterial artificial chromosomes （BAC）, using YAC and BAC as transgenic vectors may be an ideal tool because they can contain all major important elements of lacto protein. The transgenic techniques via YAC and BAC vectors may be another approach to obtain transgenic animals as mammary gland bioreactors with high efficiency and high expression without gene targeting.
作者 辛艳丽 邓继先
机构地区 军事医学科学院生物工程研究所
出处 《生物技术通讯》 CAS 2005年第5期556-559,共4页 Letters in Biotechnology
关键词 转基因动物 乳腺生物反应器 位置效应 酵母人工染色体 细菌人工染色体 transgenic animals mammary gland bioreactor yeast artificial chromosome bacterial artificial chromosome
分类号 Q953 [生物学—动物学] Q78 [生物学—分子生物学]
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参考文献26

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共引文献93

同被引文献11

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生物技术通讯
2005年 第5期

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