DcR3反义RNA与顺铂对肝癌细胞凋亡影响的比较

Comparison of cellular apoptosis processed by DcR3 antisense RNA and cisplatin in hepatocellular carcinoma

目的:比较DcR3反义RNA与顺铂对肝癌细胞凋亡的影响。方法:流式细胞术与 TUNEL等方法检测DcR3反义RNA和顺铂引起肝癌细胞凋亡的程度并进行比较。结果:DcR3 反义 RNA处理组与顺铂处理组的凋亡率与对照的单纯肝癌细胞株组比较差异有统计学意义(P<0 .05),而其二者之间凋亡率差别无统计学意义(P>0 .05)。结论:DcR3反义RNA有效降低 DcR3 表达,同时有较强的促凋亡作用,与顺铂作用相似。

Objective ： TO compare cellular apoptosis of hepatocellular cencinoma processed by DcR3 antisense RNA and cisplatin. Methods ： Measuring apoptosis stase with TUNEL, Flow cytometry,while oomloaring the alooptosls rate among 3 groups. Results： Apoptosis rates of hepatocarclnoma cellular processed by group DcR3 entisense RNA and group clsplatin were statistically hlsher than contrast group （P〈0. 05）,and there was no statistical difference between the 2 goups of DcR3 antisense RNA and clsplatin（P〉0. 05）. Conclusion ：DCR3 antlsense RNA oOUlCt antasonlze expression of DcR3, furtiqsr Induce apoptosis, and there was no statistical difference comloaring with cispatin.