丹参对梗阻性黄疸肾脏线粒体保护作用的实验研究

The protective effect of Salvia Miltiorrhizae on renal mitochondria in rats with obstructive jaundice

目的探讨氧自由基在梗阻性黄疸引发肾脏损害中的作用及丹参对肾脏线粒体的保护作用。方法将54只Wister大鼠随机分为假手术对照组(A组),胆总管结扎组(B组),胆总管结扎+丹参治疗组(C组)。采用胆总管结扎法(CBDL)建立大鼠梗阻性黄疸模型,C组应用丹参注射液5ml/(kg·d)腹腔注射治疗21d。3组大鼠分别于术后第7天(d7)、14天(d14)、21天(d21)处死,检测血清胆红素(BIL)、肌酐(Cr)、尿素氮(BUN)以及肾脏线粒体脂质过氧化产物丙二醛(MDA)和线粒体膜胆固醇含量的变化,观察肾脏及线粒体病理组织学改变,并作比较。结果B组及C组大鼠肾脏线粒体膜胆固醇及MDA含量高于对照组(P<0.05),但C组增高的程度低于B组(P<0.05),并且病理改变也较轻。结论氧自由基所引发的脂质过氧化作用是阻黄引起肾脏损害的重要原因之一,丹参可降低脂质过氧化作用对肾脏线粒体的损害作用,对梗阻性黄疸时的肾脏损害具有保护作用。

Objective：To investigate the role of oxygen free radicals in renal dysfunction in rats with obstructive jaundice and the protective effect of Salvia Miltiorrhiae（SM） on renal mitochondria. Methods： The 54 Wistar rats were randomly divided into three groups： sham-operation control group（group A, n = 18）, obstructive jaundice group（group B, n = 18）, SM-treated group（group C,n= 38）. The model of obstructive jaundice in rats was established by common bile duct ligation（OBDL）. In group C SM（5ml/kg·d） was given through abdominal cavity for 23 days. The rats were executed at 7th,14th,and 21th day after operation respectively. The contents of serum BIL,Cr, BUN and the contents of renal mitochondria MOA and cholesterol were determined respectively,and the changes of renal histopathology were observed. Results ：The contents of renal mitochondria MDA and cholesterol in group B and group C were markedly higher than group A（P〈0.05） ,and the contents of renal mitochondria MDA and cholesterol in group C were much lower than group B （P〈0. 05）,the changes of renal histopathology were minor in group Conclusion ：Free redlcals play an important role in the pathogenesis of renal lipid peroxidization injury in rats with obstructive j anudice. Salvia Miitiorrhiae can protect renal mitochondria from the impact of lipid peroxidization Injury caused by obstructive jaundice, it can protect the kidney and its function.