心肌缺血后控制性再灌注时间对再灌注损害的影响

The effect of controlled reperfusion duration on protection of ischemic reperfused hearts

家兔８０只，分成４组，每组１０只供心兔，１０只供血兔，采用在体循环灌注离体心脏模型，对心肌缺血后不同控制性再灌注（控灌）时间对再灌注损害的影响进行了研究。结果显示：５、１５和３０ｍｉｎ的控灌对心肌肌酸磷酸激酶的同工酶（ＣＰＫ－ＭＢ）、含水量（ＭＷＣ）、丙二醛（ＭＤＡ）含量以及超微结构的影响明显优于未经控灌的实验组（Ｐ＜０．０５），５ｍｉｎ和３０ｍｉｎ控灌组差异不显著（Ｐ＞０．０５），１５ｍｉｎ控灌组其各项指标又明显优于５ｍｉｎ和３０ｍｉｎ控灌组。结果提示：控灌有预防缺血再灌注损伤的作用，且以１５ｍｉｎ控灌疗效最佳。

rabbits were divided at random into 4 groups, 20 rabbits for each group (10 heart-supplying rabbits, 10 blood-supplying rabbits). The model of the isolated rabbit heart perfused with in vivo circulation was used in this study. The effect of different controlled reperfusion durations on the protection of ischemic reperfused hearts was studied.The results showed that the parameters of myocardial CPK-MB, water contents, MDA generation, and the ultrastructure of myocardium in groups of 5,15, and 30 min (Ⅱ,Ⅲ,Ⅳ) controlled reperfusion are significantly better than those in group Ⅰ(uncontrolled reperfusion) (P<0.05).No differences were found between group Ⅱ and Ⅳ (P>0.05). The parameters in group Ⅲ were significantly better than those in groups Ⅱ and Ⅳ(P>0.05 ). The results demonstrated that controlled reperfusion can reduce myocardial reperfusion injury, and that the best effect was showed in the 15 min group of controlled reperfusion. The myocardial protection of controlled reperfusion could be weakened if the duration was prolonged.