颅内出血大鼠脑组织核转录因子κB与抑制因子κBα的表达

The expression of NF-κB and IκBα in rat brain after experimental intracranial hemorrhage

目的研究实验性大鼠脑出血后脑组织核转录因子κB(NF-κB)及其抑制因子κBα(IκBα)的表达变化。方法将24只大鼠随机分为正常对照组和脑出血后6h、1d和3d实验组,每组6只大鼠。采用定量Ⅶ型胶原酶注入大鼠尾状核建立脑出血模型,用免疫组织化学法分别检测各组NF-κB及IκBα蛋白的表达。结果脑出血后6h,NF-κB蛋白表达增加,1d增加最为明显,广泛表达于血肿周围组织、远区皮质、海马等部位,并有核移位现象。在脑出血后6h、1d、3d,NF-κB吸光度值分别为0·21±0·05、0·32±0·09、0·25±0·07,与正常对照组的0·08±0·01相比,差异均有显著性(P<0·01)。在脑出血后6h、1d、3d,IκBα吸光度值分别为0·15±0·06、0·12±0·04、0·14±0·05,与正常对照组的0·30±0·07比较,IκBα表达量均减弱,差异有显著性(P<0·01)。结论NF-κB参与了脑出血后血肿周围的损害过程,而IκBα可能起到脑保护作用。

Objective To observe the expression anti significance of nuclear transcription factor-κB （ NF-κB ） and inhibitory factor κBα （1κBα） in rat brain after experimental intraeranial hemorrhage. Methods 24 Wistar rats were randomly assigned into normal group and 3 model groups. There were 6 rats in each group. The experimental intraeranial hemorrhage model was established by injecting quantitative collagenase into the left caudate nuclei of the rats. Immunohislnchemistry was used to determine the expression of NF-κB and IκBα. Results Little NF-κB positive cells were found in the normal group （gray scale value was 0. 08 ±0. 01 ）. At 6h, 1d and 3d after intraeerebral hemorrhage, the expression of NF-κB were increased signifieantly （ gray scale value was 0. 21 ±0. 05 , 0. 32 ±0. 09 , 0. 25 ±0. 07, P 〈 0. 01, respectively）. NF-κB protein expressed extensively in perihematomal tissue, cortex and hippoeampus. In addition, NF-κB nuclear translocation could be observed. At 6h, 1d and 3d, the expression of IκBα in intraeerebral hemorrhagic groups were lower than thah in the control group （0. 15 ±0. 06, 0. 12 ± 0. 04, 0. 14 ±0. 05 vs. 0. 30 ± 0. 07, P 〈 0. 01 , respectively）, Conclusion NF-κB may partieipate in the brain damage after experimental intracerebral hemorrhage, and IκBα may have potential neuroprotective effect.