血管内皮细胞生长因子及受体在乳腺癌组织的表达研究

Expression of Vascular Endothelial Growth Factor and Its Receptor in Breast Carcinoma

目的探讨血管内皮细胞生长因子(VEGF)及其受体在乳腺癌组织中表达的意义。方法使用逆转录聚合酶链反应(RT-PCR)及计算机条带分析半定量方法检测了VEGF异构体及受体mRNA在乳腺癌组织及癌周组织的表达水平。对VEGF121mRNA及KDRmRNA表达水平和肿瘤大小,淋巴结转移及绝经前后的关系进行统计学分析。结果在乳腺癌组织及癌周组织均检测到VEGF121和KDR,flt-1的mRNA表达,VEGF121和KDR在癌组织表达水平明显高于癌周组织,在癌组织及癌周组织,VEGF121mRNA表达和KDR121mRNA表达之间有明显直线正相关及回归依存关系,VEGF121,KDR的mRNA表达在肿瘤≥2cm;淋巴结阳性及绝经前组病人明显高于淋巴结阴性、肿瘤<2cm和绝经后组病人。结论VEGF、KDR在乳腺癌组织的表达水平明显高于癌周组织,VEGF积极参与乳腺癌的新生血管增殖过程。

Objective To investigate the role of vascular endothelial growth factor and its receptors during the development of progressive disease of breast cancer. Methods The mRNA expression of the alternative spliced VEGF and VEGF receptors were examined by RT - PCR in breast carcinoma and adjacent non- neoplastic breast tissues. VEGFI21 mRNA and KDR mRNA expression were analyzed together with their relation to lymph node metastasis, tumor size and hormonal status of breast carcinoma. Re,tilts 333ree VEGF transcripts were detected in beth malignant and non - malignant breast tissues. The KDR and VEGF transcripts were more abundant in the breast cancer than in the adjacent non - neoplastic breast tissues. Positive linear correlation was observed between VEGF and KDR in the breast carcinomas and adjacent non - neoplastic breast tissues. VEGF^21 and KDR mRNA expression in breast carcinomas were significandy correlated with tumor size, lymph node metastasis and menopausal. Conclusion The intense expression of VEGF, KDR mRNA in breast carcinoma provides strong evidence linking VEGF expression to the angiogenesis associated with breast carcinoma.