高脂饮食诱导的肥胖大鼠体内瘦素的变化

The Change of Leptin in Rat Obesity Induced by Lipid Rich Food

目的高脂饮食诱导的肥胖大鼠肥胖特点及体内瘦素的变化。方法利用含20%猪油的高脂饲料和普通饲料分别喂养SD大鼠20只和10只8周,建立饮食性肥胖的动物模型。用酶联免疫法测定两组大鼠血清中瘦素含量,然后对肥胖组与对照组的体重、肝、肾、脑组织的重量、胆固醇、甘油三酯、瘦素(leptin)含量进行比较。结果肥胖组大鼠体重、脑、肝、肾组织重量均>对照组,且肥胖组大鼠脑、肝、肾重量与体重呈显著正相关,p<0.01。肥胖组大鼠血清甘油三酯、瘦素含量明显高于对照组,p<0.05。结论含20%高脂饲料能够诱导部分雄性SD大鼠发展为饮食性肥胖。在肥胖组大鼠体内存在着高瘦素血症,提示有瘦素抵抗。

Objective To study the character of rat doesity and change of leptin induced by high fat diet. Methods 20 SD rats and 10 SD rats were feed with lipid rich food （20% lard） and normal food for 8 weeks to make alimentary doesity model respectively. The level of serum leptin in two group were estimated with enzyme - linked inmmo- serbent assay. The level of rat body weight , liver, kidney, weight of brain tissue ,cholesterol, trigtyceride and leptin in two groups were compared respectively. Restflts The level of rat body weight, brain, liver and kidney in doesity group were higher than that of in contrast group and the weight of brain, liver and kidney is significant positive correlation with body weight in obesity group （p 〈 0.01）. The level of senam trigtyceride and leptin in obesity group were higher than that of in contrast group（p 〈 0.05） . Conclusion It can induce alimentary obesity in part of male SD rats can be induced with continuons lipid rich food including 20% lard. High concentration of serum leptin was existed in obesity group.