海狗油保肝作用的实验研究

Effect of seal oil for protecting liver cells from acutely or chronically damaged in mice and rats

目的：观察海狗油对化学毒物所致小鼠、大鼠急性肝损伤及对长期喂食高脂饲料大鼠的影响。方法：实验分为6组(n=10)：即空白组、模型组、阳性药组、海狗油低、中、高剂量组。化学毒物乙硫氨酸或四氯化碳致急性肝损伤实验：小鼠和大鼠各组分别灌胃(ig)给供试药9d或7d；d7ig DL-乙硫氨酸，实验结束时测定肝脂；d6ip四氯化碳，实验结束时测定血清谷丙转氨酶(ALT)、谷草转氨酶(AST)和组织学检查。长期喂食高脂饲料大鼠实验：于实验d1一次性皮下注射小剂量四氯化碳并长期喂食高脂饲料造成脂肪肝模型大鼠，同时各组分别ig供试药，每天1次，连续10周后，称肝脏重量并计算肝脏系数，测定血脂、肝脂、脂质过氧化指标和组织学检查。结果：对乙硫氨酸致急性肝损伤的小鼠和大鼠ig给予海狗油(小鼠7．2 g·kg^(-1)，大鼠4．8g·kg^(-1))后，与模型组比较甘油三酯(TG)分别降低23％和16％(P＜0．01)；对四氯化碳致急性肝损伤的大鼠，海狗油4．8mg·kg^(-1)组使ALT降低24％(P＜0．01)、AST降低16％(P＜0．01)。长期喂食高脂饲料大鼠，与模型组比较，海狗油4．8mg·kg^(-1)组血清总胆固醇(TC)、游离脂肪酸(FFA)、肝重量及肝细胞内TG、TC、FFA和丙二醛(MDA)含量显著降低(P＜0．01)，超氧化物歧化酶(SOD)活性升高(P＜0．01)，组织学检查显示肝细胞内脂变程度减轻，脂滴减少。结论：海狗油对化学毒物所致急性肝损伤的肝细胞有保护作用，对脂肪肝的形成具有明显的预防作用。

protecting live or chronically AIM： To study the effect of seal oil for r cells from acutely damaged by chemicals damaged by feeding with high fat diet in mice or rats. METHODS ： KM mice and Wistar rats were divided randomly by body weight to 6 groups （n = 10 in each） including placebo control group received only olive oil, model group received olive oil too, low or medial or large dose of seal oil groups received seal oil, control drug group received dimethyl-4,4＇-dimethoxy-5, 6, 5＇, 6＇-dimethylenedioxybiphenyl-2, 2＇ dicarboxylate or simvastatin for 9, 7 days or 10 weeks. Except for placebo control group the mice or rats were acutely damaged by DL-ethionine or carbon tetrachloride on 7 or 6th day. The content of triglyceride （TG） in liver of both mice and rats or serum transaminase ALT, and AST of rats were tested in the end. The rats were chronically damaged by subcutaneous injecting a low dose of carbon tetrachloride on the first day and feeding with high fat diet for 10 weeks. The lipid levels in both serum and liver and the hepatic weight were tested and pathology was evaluated. RESULTS： while the mice and rats were damaged by DL- ethionine, the content of TG was 23% and 16% lower than model group （P〈 0.01） in the mice Of 7.2 g·kg^-1 seal oil group and in the rats of 4.8 g·kg^-1 seal oil group, respectively. In damaged rats by carbon tetrachloride, serum ALT and AST decreased 24% and 16% comparing with model group （P 〈 0.01） in 4.8 g·kg^-1 seal oil group. In chronically damaged rats by feeding with high fat diet, the total cholesterol （TC） and free fatty acid （FFA） in serum, TG, TC, FFA, malonyldialdehyde （MDA） in liver and hepatic weight decreased significantly than model group （ P 〈 0.01 ） and superoxide dismutase （SOD） in liver increased significantly than model group （P 〈 0.01 ） in 4.8 g·kg^-1 seal oil group. Pathologic histomorphology investigation showed hepatocytes with lipid droplet decreased than model group in seal oil groups. CONCLUSION： Seal oil can protect liver cells from acutely damaged by DL-ethionine or carbon tetrachloride and significant prevention effect on fatty liver.