survivin反义寡核苷酸在体外诱导胰癌细胞凋亡

Apoptosis of pancreatice cancer cells induced by antisense RNA of survivin gene in vitro

目的:探讨survivin反义核酸对胰腺癌细胞株Panc-1细胞凋亡的影响。方法:用脂质体瞬时转染法介导survivin反义核苷酸处理胰腺癌Panc-1细胞后,MTT试验测定转染细胞的相对存活率,RT-PCR检测survivin mRNA表达,琼脂糖凝胶电泳分析其对Panc-1细胞的凋亡诱导作用。结果:转染survivin反义核酸的Panc-1细胞增殖明显受抑制,与对照进行比较,具有显著性差异(P<0.05);经琼脂糖凝胶电泳,转染survivin反义核酸的Panc-1细胞可见到DNA梯形条带,而对照细胞未见到。结论:survivin反义核酸能够诱导Panc-1细胞凋亡。

Objective： To explore the effects of survivin anfisense RNA on apoptosis in pancreatic cancer cell Pane-1. Methods： survivin anfisense RNA was transferred into pancreatic cancer cell Panc-1 by lipofecfion. The opposite livability on Panc-1 cell line were assayed with MTT test. Expression of survivin detected by RT-PCR. Apoptosis was detected by DNA gel electrophoresis. Results： survivin antisense RNA obviously inhibited the Panc-1 cell growth（ P 〈0.05）. Agarose gel electrophoresis of genomic DNA from survivin anfisense RNA transferred Panc-1 showed typical DNA ladder, but DNA from other ceUs did not. Conclusion： survivin anfisense RNA can effectively induce apoptosis in Panc-1 cell.