燃煤型高氟玉米氟骨症早期大鼠血清骨钙素的变化

Change of Bone γ-Carboxyglutamic Acid Containing Protein in the Serum of Rats at the Initial Stage of Skeletal Fluorosis Caused by Fluoride in Coal

目的研究燃煤型高氟玉米氟中毒骨病变早期血清骨钙素的变化。方法选择SD大鼠,随机分6组(每组34只,雌雄各半),设对照组(正常饲料,含氟9.37mg/kg)、低氟组(含20%病区玉米,含氟38.28mg/kg)、中氟营养组(含45%病区玉米加营养,含氟51.52mg/kg)、中氟组(含45%病区玉米,含氟51.52mg/kg)、高氟营养组(含75%病区玉米加营养,含氟69.33mg/kg)、高氟组(含75%病区玉米,含氟69.33mg/kg)。分2批穴实验的第90天,高氟染毒组出现明显氟斑牙为第1批;实验的第130天,中氟染毒组出现明显氟斑牙为第2批雪以股动脉放血法处死动物,检查氟斑牙,测定尿氟浓度、骨和肾氟含量,骨密度(BMD),骨钙含量、尿钙浓度,血清骨钙素(BGP)浓度。结果建成氟中毒动物模型。各氟染毒组均出现轻度和中度氟斑牙,对照组未见氟斑牙。各氟染毒组大鼠尿氟浓度随染毒时间的延长呈上升趋势。中氟组、高氟营养组和高氟组大鼠尿氟浓度高于对照组(P<0.05或0.01)。各氟染毒组大鼠骨和肾氟含量随染毒剂量的增加呈上升趋势,一定氟染毒剂量下,营养组骨和肾氟含量低于非营养组。除第1批低氟组外,随着氟染毒剂量增加,两批各氟染毒组大鼠BMD较对照组升高(P<0.05或0.01)。与对照组比较,随着氟染毒剂量增加,两批各氟染毒组大鼠骨钙含量上升,尿钙浓度下降。各氟染毒组大鼠血清BGP浓度呈上升趋势,氟染毒第90天时,高氟组高于对照组,有统计学意义(P<0.01);氟染毒第130天时,中、高氟染毒组高于对照组,有统计学意义(P<0.01),且高于第90天时的水平(P<0.05或0.01)。结论血清BGP是反映燃煤型高氟玉米氟中毒骨病变的一个早期指标。降低摄氟量及改善营养状况,可缓解氟中毒病情。

Objective To study the change of bone γ-carboxyglutamic acid containing protein（BGP） in serum of rats at the initial stage of skeletal fluorosis caused by fluoride in coal. Methods SD rats were randomly divided into 6 groups （the number of female and male in each group was the same respectively）： the control group, the low-dose fluoride group, the middle-dose fluoride added nutrition group, the middle-dose fluoride group, the high-dose fluoride added nutrition group, the high-dose fluoride group. All rats in the experimental groups were fed on the corn collected from the prevalent areas and contained different contents of fluoride respectively for 90-100 days. Content of fluoride in the urine, bone, kidney, BGP in serum, bone mineral density （BMD） and calcium in the bone and urine were determined. Results The fluorosis of the rats became more serious as fluoride intake increased. On the condition of same fluoride intake, the fluorosis could be relieved if nutrients added. BGP in serum of rats in each experimental groups had a increase trend, at the earlier stage, BGP of the high-dose group was higher than that of the control group （P〈0.01）,at the latter days, BGP of the middle-dose, the high-dose added nutrition and the high-dose groups was higher than that of the control group （P〈0.01）, BGP of the middle-dose, the high-dose added nutrition and the high-dose groups at the latter days was higher than that at the earlier days（P〈0.05 or P〈0.01）. Conclusion BGP content in the serum is increasing at the earlier stage of skeletal fluorosis caused by fluoride in coal, which can be taken as the early indicator for skeletal fluorosis.