乙醇对心肌细胞凋亡及凋亡相关基因Bcl-2和Bax表达的影响

Effect of Alcohol on Apoptosis and Expression of Bcl-2 and Bax Genes of Myocardial Cells of Mice

目的研究乙醇对心肌细胞凋亡及凋亡相关基因Bcl鄄2和Bax表达的影响。方法将雄性昆明种小鼠随机分为4组,每组6只,分别给予10%,20%,30%的乙醇和生理盐水腹腔注射,每日1次,连续30d,通过心肌细胞的核固红鄄结晶紫和免疫组织化学染色,分析细胞凋亡及凋亡相关基因——B细胞淋巴瘤/白血病鄄2基因(B鄄celllymphoma/leukemia鄄2,Bcl鄄2)和Bcl相关蛋白(Bcl鄄associatedxprotein熏Bax)的表达。结果实验组部分心肌细胞核染色质凝集,染为蓝紫色;定量分析显示,实验组异常的心肌细胞核明显多于对照组(P<0.01)。实验组心肌细胞中Bcl鄄2的表达弱于对照组(P<0.01),且分布不均;而实验组心肌细胞中Bax的表达明显增强(P<0.01),可见棕色粗大颗粒。结论心肌细胞凋亡在乙醇对心肌影响的发生机制上发挥一定的作用;随着乙醇浓度的增加,心肌细胞的凋亡征象愈加明显;乙醇引起的心肌细胞凋亡与凋亡相关基因Bcl鄄2和Bax的异常表达有关。

Objective To study the effect of alcohol on apoptosis and apoptosis-associated genes-Bcl-2 and Bax of cardiac muscle cell. Methods We established an animal experimental model by giving different concentration of alcohol to the healthy mice. Myocardial apoptosis and the expression of apoptosis-associated gene Bcl-2 and Bax were quantitatively analysed by the nuclear fast red-crystal violet staining and immunohistochemical method. Results Some nuclears of the experimental groups were stained purple and agglutinated. Counting under the high power microscope, it was found that the amount of abnormal nuclears significantly increased in alcohol-treated groups compared with the control group （P〈0.01）. Compared with the control, the expression of Bcl-2 in all experimental groups was down-regulated （P〈0.01） and the granules were stained weak and maldistribution. In contrast, the expression of Bax was up-regulated （P〈0.01） and the granules engrained and gross. Conclusion The apoptosis of myocardial cells will play an important role in the damage of cardiac muscle induced by alcohol. The myocardial apoptosis caused by alcohol is related to the abnormal expression of apoptosis-associated gene Bcl-2 and Bax.