不同固定条件下细胞与活细胞的原子力显微镜实时观察

REAL TIME OBSERVATION ON CELLS IN DIFFERENT FIXED CONDITIONS AND LIVING CELLS IN BUFFER SOLUTION BY AFM

用原子力显微镜(atomforcemicroscope,AFM)观察固定细胞的最佳条件并在生理溶液中对活细胞实时观察。用不同固定剂和同一固定剂的不同浓度处理细胞;不加任何固定剂而直接在生理溶液中对细胞进行AFM成像。以戊二醛为固定剂并使用0.5%～1%的浓度固定细胞,后用缓冲溶液漂洗,再对细胞进行成像时可获得质量良好的图像。直接在生理溶液中进行观察,成像质量低于使用固定剂的细胞,但保持了细胞的生活原貌。在用原子力显微镜高分辨率观察生理条件下细胞的特点时,需要在制样与观测系统两方面进行改进。

Apply AFM to study the best situation observed the fixed cells in the air and the living cells in buffer solution in real time. Useing AFM to observe the cells treated with different fixative and the same fixative but different concentration as well as the cells without treated with any fixative. Imagine with good quality can be acquired when cells were treated with 0.5%-1% glutaraldehyde for 15 min then washed with Hanks solution. Thus, the imagine obtained from the living cells in the Hanks solution is poorer than that of cells fixed in the air, but they can be kept in the living state. Utilize the ATM to acquire imagines with high resolution of living cells, the improvement on the preparation of the cells and the observation system is necessary.