期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

RNA干扰的研究方法 被引量：1

Research methods of RNA interference
下载PDF
导出
摘要 RNA干扰(RNA i)技术是把与内源mRNA编码区同源的小片段外源双链RNA(doublestranded RNA)导入细胞中,导致特定基因表达沉默的一种技术。本文简要描述了RNA i的起源、原理、在动物方面的应用领域;主要叙述了RNA i技术的机制以及siRNA试验步骤,包括siRNA的设计、合成、检测方法等;比较了各种方法的特点。 RNA interference （RNAi） is a biological process in which the introduction of homologous double-stranded RNA（dsRNA） into a cell leads to targeted post-transcriptional gene silencing. This paper summarizes its origin and applied fields. The RNAi mechanism and its precedure including its design, synthesis and result test are also outlined. Merits and shortcomings of every method are compared.
作者 杨宗照 帅江冰 Mudasser Habib
机构地区 浙江大学预防兽医研究所
出处 《中国兽药杂志》 2005年第11期23-28,共6页 Chinese Journal of Veterinary Drug
关键词 RNAI SIRNA 应用 RNAi, post-transcriptional gene silencing
分类号 Q522.2 [生物学—生物化学]
  • 相关文献

参考文献20

二级参考文献121

  • 1金冬雁 黎孟枫.分子克隆实验指南[M]：第二版[M].北京：科学出版社,1993..
  • 2[14]Jacque JM,Triques K,Stevenson M.Modulation of HIV-1 replication by RNA intference[J]. Nature,2002,418:435-438.
  • 3[15]Elbashir SM,Harborth J,Weber K, et al.Analysis of gene function in somatic mammalian cells using small interfering RNAs [J].Methods,2002,26:199-213.
  • 4[1]Fire A, Xu S,Montgmery MK, et al.Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans[J].Nature,1998,391:806-811.
  • 5[2]Berstein E,Caudy A,Hammond SM, et al.Role for a bidentate ribonuclease in the initiation step of RNA interference[J].Nature,2001,409:363-366.
  • 6[3]Wassenegger M,Pelissier T.A model for RNA-mediated gene silencing in higher plant [J]. Plant Mol Biol,1998,37:349-362.
  • 7[4]Sayda ME,Jens H,Winfried L, et al.Duplexes of 21-nucleotide RNAs mediate RNA interference in all cultured mammalian cells[J].Nature 2001,411:494-498.
  • 8[5]Nykanen A,Haley B,Zamre PD.ATP requirements and small interfering RNA structure in the RNA interference pathway[J]. Cell,2001,107:309-321.
  • 9[6]Michael T,McManus,Phillip AS.Gene silencing in mammals by small interfering RNAs[J].Nature Reviews Genetics,2002,3:737-747.
  • 10[7]Harborth J,Elbashir SM,Bechert K, et al.Indentification of essential genes in cultured mammalian cells using small interfering RNAs[J].Cell Sci,2001,114:4557-4565.

共引文献77

同被引文献12

  • 1崔秀云.核糖核酸酶抑制因子[J].生物工程进展,1996,16(5):17-20. 被引量：7
  • 2Schneider R, Higgins M J, Kieninger D, et al. The Human ribonu- clese angiogenin inhibitor is encoded by a gene mapped to chromo- some 11 p15.5, within 90kb of the H-RAS protooncogene[ J]. Cy- togenet Cell Gene, 1992,59(4) :264-267.
  • 3Lee Fs, Shapiro R, Vallee BL. Tight-Binding inhibition of angio- genin and ribonuclease A by placental fibonuclease inhibitor [ J ].Biochemistry, 1988,28( 1 ) :225-227.
  • 4Lee Fs, Auld DS, Vallee BL. Trptophan fluorescence as a probe of placental ribonuclease inhibitor binding to angiogenin [ J ]. Bio- chemistry, 1989,28( 1 ) :219-210.
  • 5Cui XY, Fu PF, Pan DN, et al. The antioxidant effects of ribonu- clease inhibitor[J]. Free Radie Res, 2003,37(10) :1079-1085.
  • 6李坤.人核糖核酸酶抑制因子干涉载体的构建及其对肿瘤细胞生长的影响[D].博士学位论文,2008.
  • 7Franceschini IA, Feigenbaum-Lacombe V, Casanova P, et al. Ef- ficient gene transfer in mouseneural precursors with a bicistronic retroviral vector [ J ]. J Neurosci Res, 2001,65 ( 3 ) : 208 -219.
  • 8Zhang J, Liu W, Liu J, et al. G-protein 152 subunit interacts with mitofusin 1 to regulate mitochondrial fusion [ J ]. Nat Commun, 2010,1:101.
  • 9Xia H, Mao Q, Paulson H, et al. siRNA-mediated gene silencing in vitro and in vivo [ J ]. Nature Biotechnology, 2002,20 ( 10 ) : 1006-1010.
  • 10邹文远,张自力,胡道予.血管内皮生长因子与原发性肝癌血管新生关系及抗血管治疗的进展[J].放射学实践,2009,24(3):338-340. 被引量：3

引证文献1

中国兽药杂志
2005年 第11期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部