铁皮石斛组培品与野生品的形态组织学和多糖含量比较研究

A comparison of tissue formation and the content of polysaccharide between wild and cultured Dendrobium candidum

目的:比较铁皮石斛组培品和野生品在植物形态、组织结构及总多糖含量上有无差异。方法:显微切片观察、显微摄影;苯酚-硫酸法测定多糖含量。结果与结论:铁皮石斛组培品与野生品在植物形态及组织结构上基本相同,总多糖含量无显著差异,表明铁皮石斛经组织培养和人工培植过程后药材性状和质量与野生药材相近,初步证明经过组织培养快速繁殖和人工培植铁皮石斛是解决其资源紧缺问题的有效途径。

Objecetive： To compare the tissue formation and the content of polysaccharide between the wild Dendrobium candidum and the cultured ones and to find any existed differences. Method： Bare-handed microtomy and photomicrography; The content of polysaccharide is determined by phenol-sulphuric acid method. Result and conclusion： There are no marked noticeable differences between the wild D. candidum and the cultured ones in terms of the fissure formation and the content of polysaccharide.