美国小麦品种高分子量麦谷蛋白亚基组成分析

Composition Analysis of HMW-GS in American Wheat Cultivars

为了给我国小麦品质育种工作提供有益的信息,利用SDS-PAGE技术对美国20世纪90年代128份主要推广小麦品种的高分子量谷蛋白亚基(HMW-GS)组成进行了研究.结果表明,在参试材料中共检测到15种不同的亚基类型.在Glu-A1位点有Null、2*、1和一个未知亚基4个等位变异类型,以Null为主要类型;Glu-B1有7、7+8、7+9、6+8、13+16、14+15、17+18等7个等位变异类型,以7+9为主要类型;Glu-D1有2+12、5+10、2+10、2+未知亚基4个等位变异类型,以5+10为主要类型.同时发现共有27种亚基组合,以'2*,7+9,5+10'为主要类型.所有品种品质得分范围为4～10,平均得分为7.5.研究表明供试品种品质普遍较好.

One hundred and twenty-eight wheat cultivars from America were analyzed by SDS-PAGE to determine their high molecular weight（HMW）glutenin subunit combinations. A total of fifteen alleles were identified at Glu-1. Glu-A1 encoded four subunits, 1, 2^＊, Nulland an unidentified subunit, and Null was the major type; Glu-B1 encoded seven subunits, 7, 7＋8, 7＋9, 6＋8, 13＋16, 14＋15, 17＋18, and 7 ＋9 was the major type; Glu-D1 encoded four subunits, 2＋12, 5＋10, 2＋10, 2＋ an unidentified sub- unit, and 5＋ 10 was the major type. Furthermore, twenty-seven subunit combinations were defected, and the composition of “2^＊, 7＋9,5＋10”was the major type. Payne scores ranged from 4 to 10, with an average of 7.5. Therefore the quality of these varieties were good.