摘要
建立了偶氮胂Ⅲ作显色剂比色测定血清钙的方法。缓冲溶液为ｐＨ＝７．０的三乙醇胺－盐酸缓冲系，工作波长为６６０ｎｍ，线性０～４．８０ｍｍｏｌ／Ｌ，检测限为０．１１ｍｍｏｌ／Ｌ，批内、批间变异系数分别为３．２％、４．１％。方法简便，稳定，精确度好，选择性高，不受标本溶血、黄疸、脂血的影响。
We described a method using the single reagent arsenazo Ⅲ for thespectrophotometric determination of calcium in serum. The triethanolamine -hydrochloric acid buffer used was pH7.0 and the working wavelength for photometry was 660nm. The standard curve for calcium was linear from 0 tO 4.80mmol/L and the limit of detection was 0. 11mmol/L. The within-run CV and between-run CV for normal serum were 3.2% and 4.1%, respectively. The method was found to be simple and reliable, and rid of the possible interference brought about by icteric, hemolyzed and lipemic samples. It was also shown that the mean values of calcium in 2 quality-control sera determined by this method well matched those by other standard methods.
出处
《徐州医学院学报》
CAS
1996年第1期26-28,共3页
Acta Academiae Medicinae Xuzhou
