摘要
采用Fmoc法固相合成胸腺五肽(TP-5)。利用RP-HPLC和质谱对其进行鉴定,绘制胸腺五肽纯品的标准曲线,作为胸腺五肽的准确定量方法。考察不同的反应溶剂、缩合剂和切割条件对合成胸腺五肽纯度的影响,并由此确定最佳合成工艺条件:以DMF为反应溶剂,HATU为缩合剂,TFA、p-甲基苯酚、EDT和H2O体积比为97.00∶0.25∶0.25∶2.50溶液为切割条件。在此条件下,胸腺五肽的纯度达到80.34%。
Thymopentin (TP-5) has been synthesized by solid-phase method, and determined by reversedphase high performance liquid chromatography and mass spectrum. The standard curve of TP-5 has been drawn, and used as a method to exactly analyze the purity of crude TP-5. The influence of different coupling solvents, coupling reagents and cleavage conditions on the purity of TP-5 is reviewed. The optimized protocol is ascertained:DMF is used as the coupling solvent;HATU as the coupling reagent;and TFA:p-methyl phenol: EDT: H2O = 97 : 0. 25 : 0.25 : 2.5 as the cleavage condition. Under this condition, the final purity of crude TP-5 reached 80.34 % .
出处
《化学工业与工程》
CAS
2005年第6期407-410,共4页
Chemical Industry and Engineering
基金
天津市自然科学基金(993804311)
关键词
胸腺五肽
固相合成
RP-HPLC
标准曲线
thymopentin (TP-5)
solid-phase synthesis
reversed-phase high performance liquid chromatography (RP-HPLC)
standard curve