摘要
为了探讨柯萨奇 B 组病毒感染与云南慢型克山病的关系,本文用缺口平移法制备生物素标记的柯萨奇 B_3病毒的 cDNA 探针与云南慢型克山病尸检心肌组织15例及脑外伤等非正常死亡的尸检心肌组织10例石腊切片及引产胎儿心肌组织10例的石腊切片进行原位杂交。结果云南慢克16例中有11例出现阳性杂交信号,即有柯萨奇 B 组病毒(CVB)RNA 存在,阳性率达73.3%。脑外伤等非正常死亡的心肌及胎心均为阴性。提示云南慢型克山病与 CVB 的感染高度相关。
We performed hybridization in situ to examine RNA of Coxsackie Virus B(CVB)in paraffin-em- bedded myocardial specimens from 15 patients with chronic Keshan disease in Yunnan Province,10 pa- tients with cerebral trauma and 10 fetuses from non-endemic areas using the CVB_3c DNA probe la- belled with Bio-11-dUTP by nick translation.OF the 15 patients with chronic Keshan disase,11(73. 3%)showed positive findings.Their myocardial tissues were infected by CVB previously.The results suggested that CVB infeetion may be highly related with chronic Keshan disease in Yunnan province
出处
《中国地方病学杂志》
CAS
CSCD
1996年第2期65-67,共3页
Chinese Jouranl of Endemiology
基金
国家自然科学基金资助课题 批号 3937065
关键词
柯萨奇B组病毒
克山病
原位核酸杂交
Coxsaekie Virus B
hybridization in situ
chronic keshan disease
