摘要
目的观察人参炔醇(panaxynol,PNN)对大鼠主动脉平滑肌细胞(RASMC)增殖的抑制作用及机制。方法由细胞计数、[3H]TdR参入试验确定细胞增殖率,采用分子探针Fura-3/AM及共聚焦显微镜检测胞内游离Ca2+浓度([Ca2+]i),RT-PCR方法观察线粒体转录因子1(m tTF1)mRNA的表达。结果PNN以浓度依赖方式抑制血清及PDGF-BB诱导的RASMC增殖和DNA合成;9μmol.L-1PNN预处理RASMC能抑制PDGF-BB引起的[Ca2+]i升高;PDGF-BB上调RASMC m tTF1 mRNA表达,该作用可被3、9μmol.L-1的PNN抑制。结论PNN具有抗RASMC增殖作用,该作用与降低[Ca2+]i和抑制m tTF1 mRNA表达有关。
Aim To evaluate the inhibitory effect of panaxynol （PNN） on the proliferation of rat aortic smooth muscle cell （RASMC） and its mechanisms. Methods Cell proliferation was determined using cell count and [ ^3H ]-TdR incorporation test. Fura-3/AM and confocal were used to measure intracelluar free Ca^2＋ concentration. Expression of mitochondrial transcription factor 1 （mtTF1） mRNA was tested by using RT-PR. Results PNN inhibited the RASMC proliferation and DNA synthesis induced by serum and PDGFBB in a dose-dependent manner. 9 μmol · L^-1 of PNN inhibited the increase of intracelluar free Ca^2＋ concentration induced by PDGF-BB. PDGF-BB upregulated the expression of mtTF1 mRNA, which could be suppressed by 3, 9 μmol·L^-1 of PNN significantly. Conclusions PNN can inhibit RASMC proliferation significantly, which might be related to the decrease of intracellular free Ca^2＋ concentration and mtTF1 mRNA expression
出处
《中国药理学通报》
CAS
CSCD
北大核心
2005年第11期1313-1319,共7页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No30371731)
上海第二医科大学基金项目(No2003HZJJ0020)
