BCPT对慢性应激抑郁大鼠脂质过氧化及免疫功能的影响

Effect of BCPT on antioxidization and lymphocyte proliferation in chronic unpredictable mild stress model of depression in rats

目的观察一种拟青霉代谢物提取物(BCPT)对慢性轻度不可预见性应激(CUMS)大鼠脂质过氧化及免疫功能的影响。方法采用CUMS法,造成大鼠抑郁模型,按试剂盒说明书要求测定大鼠血清GSH-PX、CAT、T-SOD活力和MDA、NO含量。采用MTT法检测CUMS大鼠血清对正常小鼠脾淋巴细胞转化的影响。结果BCPT提高CUMS大鼠血清GSH-PX、CAT、T-SOD活力,降低CUMS大鼠血清MDA、NO含量,改善CUMS大鼠血清对小鼠脾淋巴细胞的抑制作用。结论BCPT的抗抑郁作用可能与增强CUMS大鼠的抗氧化和免疫功能有关。

Aim To study the effect of bioactive compounds from paecilomyces tenuipes（BCPT） on antioxidization and lymphocyte proliferation in chronic unpredictable mild stress （CUMS） model of depression inrats, Methods Depression animal model was reproducted in CUMS, UV spectrophotometer analysis technique was used to detect the activity of SOD、GSH-PX and CAT,and content of MDA and NO in rat serum;lymphocyte proliferation was tested with MTF method. Results BCPT could obviously enhance the activities of SOD ,GSH-PX and CAT,and also significantly inhibit the increase of MDA and NO content in serum in CUMS rats;BCPT decreased the serum from depressive rat inhibited ConA-induced lymphocyte proliferation.Conclusion BCPT produces an antidespressant-like effect in antioxidation and cellular immunological function in CUMS rats.