革兰阴性杆菌耐季铵盐消毒剂-磺胺复合物基因qac E-sul1的检测

Detection of qacE-sul1 Gene Resistant to Quaternary Ammonium Compounds and Sulphonamide Compounds in Gram Negative Bacteria

目的检测革兰阴性菌中耐季铵盐消毒剂磺胺复合物的qacEsul1基因,为临床提供细菌对消毒剂的耐药信息。方法使用稀释计数法测定60株革兰阴性杆菌对季胺盐复合物(QACs)的最低有效杀菌浓度,利用改良的表型筛选试验检测耐QACs的表型,采用聚合酶链反应(PCR)试验检测耐季铵盐消毒剂磺胺复合物基因qacEsul1。结果部分革兰阴性杆菌临床分离株对QACs的最低有效杀菌浓度,超过其推荐消毒浓度的8倍;60株革兰阴性杆菌中表型筛选试验14株阳性;11株qacEsul1基因阳性,其中部分菌株进行全长基因测序并登录美国核酸数据库(GenBank),注册号:AY769739。结论在临床常见革兰阴性杆菌中已出现由遗传物质介导的高耐QACs的细菌;本研究所采用的改良表型筛选试验能有效地检测高耐QACs的细菌表型。

OBJECTIVE To investigate qacE-sull gene resistant to the quaternary ammonium compounds （QACs） and sulphonamide compounds in Gram negative bacteria in order to provide the information of bacterial resistance to disinfectants. METHODS The minimal effective bactericidal concentration of 60 strains of Gram negative bacteria resistant to QACs was detected by diluting-counting method; the phenotype to QACs by the improved phenotype screening test, the qacE-sull gene of resistance to QACs and sulphonamide compounds by PCR. RESULTS The minimal effective bactericidal concentration of some Gram negative bacteria resistant to QACs was 8 times more than the recommending concentration. From 60 Gram negative bacteria strains 14 were positive by phenotype screening test; the qacE-sall genes of 11 strains were positive by PCR, the full sequence of some of which was registered in GenBank （Accession No. AY769739）. CONCLUSIONS The bacteria of high resistance to QACs are mediated by genetic material. The improved phenotype screening test can effectively screen the bacteria of high resistance to QACs.