白藜芦醇拮抗庆大霉素耳毒性的实验研究

Resveratrol Protects Against Gentamicin Ototoxicity

目的观察白黎芦醇(resveratrol,Res)对抗庆大霉素耳毒性的作用。方法将豚鼠随机分为庆大霉素(gentamicin,GM)组、白藜芦醇剂量I+GM组(ResI)、白黎芦醇剂量II+GM组(ResII)、白黎芦醇剂量III+GM组(ResIII)及对照组。采用听性脑干反应(ABR)、耳蜗铺片及透射电镜技术,观察用药前后各组动物听阈及耳蜗毛细胞形态学改变,并检测血清丙二醛、超氧化物岐化酶含量、肾功能以及庆大霉素血药浓度。结果ResIII组血液中丙二醛较GM组明显减少(P<0.05),GM+Res各组超氧化物歧化酶活性均明显高于GM组(P<0.05),同时GM组1、8kHz ABR4周平均阈移与ResIII剂量组间差异显著(P<0.05)。形态学改变与听力变化一致。Res对庆大霉素血药浓度没有影响。结论大剂量Res能有效减轻GM的耳毒性作用,且不影响庆大霉素的抗菌作用。

Objective To study the prevention of gentamicin ototoxicity by resveratrol（Res） in guinea pigs. Methods Auditory brainstem response and cochlear preparation transmission electron microscope were used to evaluate the effects of hair cell on hearing threshold and morphology of hair cell. Serum levels of GM were also tested. It was comprised of five groups, each group had eight animals. GM and Res were tested alone and in combination, besides control group. Results GM group developed a progressive threshold shift. Injury in high frequency was significantly more severe than that in low frequency （ P 〈 0.05 ）. Morphological results were consistant with ftmctional results. Res did not affect serum levels of GM. Conclusion These results suggest that Res may be a promising therapeutic agent to gentamicin ototoxicity.