TTV核酸和抗体在肝病患者中的检测及临床意义

The Detection and the Clinical Significance of TTV Nucleic Acid and IgG Antibody in the Patients with Hepatic Diseases

本文采用聚合酶链反应(PCR)和酶联免疫吸附试验(ELISA)分别检测了116例各型肝病患者和105例献血员血清中的TTV-DNA和TTV-IgG,结果显示两种方法的检出率有较大的差异,PCR法的敏感度和重复性均优于ELISA法。TT病毒(TTV)在肝病患者中的阳性率显著高于献血员(P<0.05),提示TTV与肝脏疾病有相关性。

T V-DNA and TTV-IgG of the serums of the patients with hepatic diseases （116cases） and the blood donors（105cases） were detected simultaneously by polymerase chain reaction （PCR） and enzyme-linked immunosorbent assay（ELISA）. The results showed that the sensitivity and the repeatability of the PCR methed was better than ELISA. And the positive rate of TTV in the patients with hepatic diseases was significantly higher than that of the blood donors （P 〈 0. 005）, which indicated that TTV was responsible for the hepatic diseases.