二期梅毒患者外周血淋巴细胞凋亡及其免疫表型的研究

Apoptosis and immunophenotype of peripheral blood lymphocytes in secondary syphilis

目的:观察二期梅毒患者外周血淋巴细胞(PBLC)凋亡及其免疫表型,探讨二期梅毒患者免疫功能异常与淋巴细胞凋亡的关系。方法:采用流式细胞仪检测33例二期梅毒患者和30例正常人PBLC免疫表型与细胞凋亡。结果:梅毒患者外周血Th细胞及Th/Ts比值明显低于对照组(P<0.01),而Ts细胞则显著高于对照组(P<0.01)。B淋巴细胞两组间的差异无显著性(P>0.05)。梅毒患者PBLC及CD4+细胞凋亡率显著高于对照组(P<0.01),CD8+及CD19+细胞凋亡率两组比较差异无显著性(P>0.05);梅毒患者PBLC凋亡率与CD4+细胞及CD4+/CD8+比值呈显著负相关(r=-0.675,P<0.01;r=-0.782,P<0.01),CD4+细胞凋亡率与CD4+细胞数呈显著负相关(r=-0.813,P<0.01)。结论:二期梅毒患者存在细胞免疫功能抑制,其细胞免疫功能异常可能与淋巴细胞凋亡过度有关。

Objective： To investigate the correlation of apoptosis with immunophenotypes of peripheral blood lymphocyte （PBLC） in secondary syphilis. Methods- PBLC immunophenotype and apoptosis in 33 cases of secondary syphilis and 30 normal controls were determined by flow cytometry （FCM）. Results： Compared with controls, Th cells and the ratio of Th/Ts were significantly decreased（ P 〈 0.01 ）, whereas Ts cells were remarkably increased in syphilitics（ P 〈 0.01 ）. There was no significant difference in B lymphocytes between both groups（ P 〉 0.05）. Apoptosis of PBLCs and CD4^＋ cells in syphilitics was superior to those in controls （ P 〈 0.01 ）. Apoptosis of CD8^＋ and CD19^＋ cells in syphilitics was not different from those in controls （P 〉 0.05）. There was a negative correlation of PBLE apoptosis with CD4^＋ cells and the ratio of CD4^＋/CD8^＋ （ r = - 0.675, P 〈 0.01 ; r = - 0. 782, P 〈 0.01 ） in syphilitic patients, and so was between CD4^＋ cell apoptosis and CD4^＋ cells（ r = - 0.813, P 〈 0.01 ）. Conclusion： There is a prominent suppression of celllar immunity in patients with secondary syphilis, which is associated with the excessive apoptosis of PBLCs.