过氧化氢酶过度表达对血管平滑肌细胞凋亡的影响

The Effect of Overexpression of Catalase on Apoptosis of Vascular Smooth Muscle Cells

目的探讨腺病毒载体介导的过氧化氢酶基因转染对体外培养的人血管平滑肌细胞凋亡的影响。方法用含过氧化氢酶基因的重组腺病毒转染人血管平滑肌细胞,采用Westernblot方法检测血管平滑肌细胞过氧化氢酶的表达,应用流式细胞术、TUNEL法等方法检测血管平滑肌细胞的凋亡。结果含过氧化氢酶基因的重组腺病毒转染后血管平滑肌细胞过氧化氢酶表达明显增多;流式细胞术显示含过氧化氢酶基因的重组腺病毒组与对照组比较凋亡率明显增加(P<0.01)。经TUNEL分析显示,含过氧化氢酶基因的重组腺病毒组凋亡细胞明显多于对照组,两者之间有显著性差异(P<0.001)。结论腺病毒载体介导的过氧化氢酶基因转染导致过氧化氢酶过度表达,促进人血管平滑肌细胞的凋亡,这可能是防治经皮腔内冠状动脉血管成形术后再狭窄的一种新方法。

Aim To investigate the effect of infection with adenoviral vector containing cDNA for human catalase on apoptosis of cultured human vascular smooth muscle cells （VSMC）. Methods Human VSMC were infected with catalase -conrained recombinant adenovirus （AdCat） in vitro, Catalase protein was detected by Western blot analysis. The VSMC apoptosis was determined by flow cytometry and TUNEL assay. Results The expression of catalase in VSMC infected with AdCat was significantly higher than that in uninfected VSMC. The number of TUNEL-labeled nuclei in AdC, at-infeeted cells was greater than that in AdLacZ-infected or noninfected cells. The percentage of apoptosis cells in VSMC infected with AdC, at was higher than that in control group （ P 〈 0.001 ）. Conclusion These findings indicate that overexpression of Catalase promoted apoptosis in VSMC. It could be a new target to prevent the restenosis after percutaneous trans luminal coronary angioplasty .