2型糖尿病患者动脉粥样硬化动脉内膜巨噬细胞炎性蛋白1α的改变

Expression and Distribution of Macrophage Inflammatory Protein-1α in Atherosclerotic Tunica Intima of Artery From Type 2 Diabetics

目的探讨巨噬细胞炎性蛋白1α在2型糖尿病患者和非糖尿病患者动脉粥样硬化动脉内膜中的表达与分布。方法采用免疫组织化学方法分别检测13例2型糖尿病患者动脉粥样硬化动脉内膜(5例脂纹期动脉内膜和8例纤维斑块期动脉内膜)、15例非糖尿病患者动脉粥样硬化动脉内膜(6例脂纹期动脉内膜和9例纤维斑块期动脉内膜)及7例健康人正常动脉内膜巨噬细胞炎性蛋白1α的表达和分布,并利用计算机图像分析仪对免疫组织化学染色切片进行灰度扫描,作相对定量分析。结果正常动脉内膜未见巨噬细胞炎性蛋白1α阳性表达,平均灰度值为234.27±8.04。巨噬细胞炎性蛋白1α在非糖尿病患者不同期的动脉粥样硬化动脉内膜中呈不同程度的表达,巨噬细胞炎性蛋白1α在脂纹期动脉内膜平均灰度值为168.40±7.69,主要分布在内皮细胞及泡沫细胞中;巨噬细胞炎性蛋白1α在纤维斑块期动脉内膜平均灰度值为173.67±6.56,主要分布在泡沫细胞,而内皮细胞仅有弱的巨噬细胞炎性蛋白1α染色。在糖尿病患者脂纹期及纤维斑块期动脉内膜中,内皮细胞及泡沫细胞中皆有较强的巨噬细胞炎性蛋白1α染色,脂纹期动脉内膜巨噬细胞炎性蛋白1α平均灰度值为132.73±6.01,纤维斑块期动脉内膜巨噬细胞炎性蛋白1α平均灰度值为138.68±9.41。结论正常动脉内膜未见巨噬细胞炎性蛋白1α阳性表达。巨噬细胞炎性蛋白1α在非糖尿病患者脂纹期动脉内膜中的表达明显增多,在纤维斑块期动脉内膜中的表达逐渐减弱。巨噬细胞炎性蛋白1α在糖尿病患者脂纹期动脉内膜中的表达显著增高,与非糖尿病患者脂纹期动脉内膜中的表达相比有显著性差异(P<0.05);在糖尿病患者纤维斑块期动脉内膜中仍有较强的表达,与非糖尿病患者纤维斑块期动脉内膜中的表达相比有显著性差异(P<0.05)。

Aim To deteet the expression and distribution of macrophage inflammatory pntein-1α （ MIP-1α） in atherosclerotic tunica intima of artery from type 2 diabetics and non-diabetics in order to try to find out the effect of MIP-1α on the formation and development of atherosclerosis in type 2 diabetics. Methods We used the immunohistochemisty method to detect the expression and distribution of MIP-1αn atherosclerotic tunica intima of artery from 13 type 2 diabetics, 15 non-diabetics and normal tunica intima of artery from 7 healthy people, respectively. Then computer image analyzer was used in relative quantu analysis. Results MIP-1α was absent in normal arteries with average gray value of 234. 27 ± 8.04 . There was different expression of MIP-1α in different-stage tunica intima of artery from non-diabetics with atherosclerosis： With the progression of atherosclerosis, the immunoreactivity of MIP-1α increased with average gray value of 168.40 ± 7.69 in fatty steaks and 173.67 ± 6.56 in fibrous plaques. MIP-1α as mainly in endothelial cells and foam cells during fatty steaks, while MIP-1αas mainly in foam cells and there was little expression in endothelial cells during fibrous plaques. Average gray value of MIP-1α in fatty steaks from type 2 diabetics was 132.73 ± 6.01. There was a significant difference between average gray value in fatty steaks from non-diabetics and average gray value in fatty steaks from type 2 diabetics （ P 〈 0.05 ）. Average gray value of MIP-1α in fibrous plaques from type 2 diabetics was 138.68 ± 9.41. There was a significant difference between average gray value in fibrous plaqus from non-diabetics and average gray value in fibrous plaques from type 2 diabetics （P〈0.05）. There were strong expression of MIP-1α in endothelial cells and foam cells during two stagcs of type 2 diabetics. Conclusion MIP-1α was aheent in normal afteries. Expression of MIP-1α in fatty steaks from non-diabetics increased obviously, but expression of MIP-1α in fibrous plaques from non-diabetics decreased graduaLly. Expression of MIP-1α in fatty steaks from diabetics increased significantly and there was a significant difference between average gray value in fatty steaks from non-diabetics and average gray value in fatty steaks from type 2 diabetics （ P 〈 0.05）. There was still strong expression of MIP-1α in fibrous plaques from type 2 diabetics and there was a significant difference between average gray value in fibrous plaques from non-diabetics and average gray value in fibrous plaques from type 2 diabetics （P 〈 0.05）.