鼻腔灌流实验在变应性鼻炎大鼠模型中的应用

Experimental System Measuring Nasal Vascular Permeability in Allergic Rhinitis Model with Brown Norway and Sprague-Dawley Rat

目的观察药物对变应性鼻炎的疗效,建立一种客观有效的大鼠鼻腔灌流模型。方法采用腹腔注射卵蛋白(OVA)和氢氧化铝凝胶混悬液,使大鼠致敏;第18天进行鼻腔灌流,测定灌流液中的伊文氏蓝的浓度。结果BN大鼠模型组灌流液中伊文氏蓝浓度高于对照组(P<0.05);SD大鼠模型组和对照组灌流液中伊文氏蓝浓度无差异(P>0.05)。结论鼻腔灌流实验结果在BN大鼠鼻炎模型中优于SD大鼠大鼠鼻炎模型。

Objective To establish a useful experimental system for evaluating the effect of drugs on allergic rhinitis. Method An animal disease model of allergic rhinitis was developed with Brown Norway rat and Sprague-Dawley rat, immunized with ovalbumin （OVA） emulsified in AI（OH）3 adjuvant. The nasal vascular permeability was assessed by determining the extent of the Evan＇blue released into the nasal perfusate following the OVA challenge to the nasal cavity of rats. Results The release of Evan＇blue following perfusion significantly increased in the sensitized BN rats and the released extent of Evan＇blue was lower in the sham-sensitized BN rats. But there was no same result in SD rats. Conclusion The vascular permeability is accelerated by the immunized antigen of OVA in the sensitized BN rats and differs from that of sham-sensitized BN rats. Measurement of the extent of nasal vascular permeability in allergic rhinitis model can be considered as a useful experimental system for evaluating drug effects on allergic rhinitis. BN rat are more sufficient than SD rat in this experiment.