青钱柳种皮甲醇浸提液的生物测定

The bioassay of the methanol extract from Cyclocarya paliurus seed coat

通过对青钱柳〔Cyclocarya paliurus(Batal.)Iljinskaja〕种皮甲醇浸提液的生物测定,探讨青钱柳种子休眠与种皮内源抑制物质的关系。结果表明,青钱柳种皮不同浓度的甲醇浸提液(10%、20%、25%、30%和40%)对白菜(Brassica campestrisL.ssp.chinensis)种子发芽率和种子活力以及幼苗的高生长、根生长均有抑制作用,浓度越高抑制作用越明显。用30%和40%浸提液处理,白菜种子不能萌发;用10%、20%和25%浸提液处理,白菜种子的萌发率分别为98.6%、75.0%和9.0%;苗高和根长分别比对照降低了31.6%、48.1%、79.7%和21.3%、70.8%、90.2%。表明青钱柳种子的种皮中含有抑制种子萌发和幼苗生长的成分。

The bioassay of the methanol extract from Cyclocarya paliurus （ Batal. ） Iljinskaja seed coat was studied in order to investigate the relationship between the restraining substances in seed coat and the dormancy of C. paliurus seed. The results showed that the methanol extract from C. paliurus seed coat in different concentration of 10%, 20%, 25%, 30%, 40% all exhibited the inhibitory role on the germinntion rate and vigor index of seed （SVI） and height and root growth of seedling of cabbage （Brassica campestris L. ssp. chinensis）, with the higher the extract concentration, the more obvious the inhibitory role. The cabbage seed could not germinate with 30% and 40% methanol extracts. The germination rate of cabbage seed treated by 10%, 20% and 25% methanol extracts were 98.6%, 75.0% and 9.0% respectively, the height and root length of cabbage seedling decreased by 31.6% , 48.1% , 79.7% and 21. 3%, 70. 8% , 90.2% respectively in comparison with the control. It is concluded that the seed coat of C. paliurus contains some germination-restraining components.