摘要
用显微注射法将Epstein-Bar病毒(EBV)的潜伏膜蛋白(LMP)基因分组注入1239只昆明种小白鼠受精卵的原核中。结果出生了25只原代鼠(Founder),其中2只基因组中整合有LMP基因,整合率为8%(2/25)。培养基的质量、每次注射的持续时间及注入基因的浓度等均影响注射效果。此外,将溶解DNA的缓冲液注入4l3只受精卵中,结果发现注入的LMP本身对注射后受精卵的存活率及幼仔出生率均有一定负性影响。
n order to study the function of nasopharyngeal carcinoma related gene-latent membraneprotein(LMP) gene of EB virus in human body,the male pronuclei of 1239 zygotes from Kun-ming white mice have been microinjected with LMP gene. Twenty-five mice have been born afterimplantation and the genomic DNA of 2 mice had the exogenous LMP gene integrated.The in-tegrated rate was 8%,Several factors having various effects on the final results,such as qualityof culture medium,duration of microinjection and concentration of injected gene,and so on, havebeen discussed.
出处
《湖南医科大学学报》
CSCD
1996年第1期1-4,共4页
Bulletin of Hunan Medical University
基金
国家"八.五"攻关
中华医学基金