不孕和生育不良结局与妇女沙眼衣原体感染

Association between abnormal reproductive outcomes and chlamydia trachomatis infection identifed by quantitative real-time F-PCR

目的 研究不孕及孕产不良与妇女沙眼衣原体感染关系。 方法 筛选妇女具 有不孕者61例、自发流产47例、宫外孕史者18例共126例,均伴有生殖泌尿系统感染症状,同 时从正常人群中随机抽取30例做对照组,取材为宫颈柱状上皮细胞,应用荧光定量PCR方法测 定病原体的感染情况: 结果 不孕组、自发流产组、宫外孕组CT阳性检出率分别为29．51％ (18／61)、31．91％(15／47)、44．44％(8/18),明显高于对照组6．67％(2／30),差异有显 著性(P<0．01,P<0．005) 结论 沙眼衣原体是造成妇女生殖异常韵主要病原体,CT荧光定 量PCR方法是对具有生殖异常史的妇女进行沙眼衣原体检测的首选方法。

Objective To investigate the relationship between Chlamydia Trachomatis infection and abnormal reproductive outcomes. Methods A total of 126 women with an abnormal reproductive history （infertility, 61 cases; spontaneous abortion, 47 cases; ectopic pregnancy, 18 cases）with symptoms of reproductive and urinary tract infection and 30 randomly selected controls were enrolled. Epithlia samples were collected from the cervix with a cotton swab, and were tested for Chlamydia Trachomatis by real-time quantitative detection PCR. Rsults The positive rates among women with fertility（29.51%）, spontaneous abortion （31.91%） and ectopic pregnancy （44.44 %） were significantly higher than that of the control group （6.67 %）. Conclusion Chlamydia Trachomatia infection is associated with selected abnormal reproductive outcomes, and real-time quantitative F-PCR can be considered as the primary choice in detecting Chlamydia trachomatis infection in clinical setting.