survivin和p53在食管鳞癌中的表达及意义

Expression and significance of survivin and p53 in esophageal squamous carcinoma

目的探讨凋亡抑制因子survivin和p53在食管鳞癌(ESC)组织中的表达及其与临床病理因素之间的关系。方法应用免疫组织化学S-P法和PowerVisionTM-9000(PV-9000)法检测60例ESC、15例食管良性肿瘤和10例癌旁正常食管黏膜组织中survivin和p53的表达。结果60例ESC中survivin和p53的阳性表达率分别为71.7%和65.0%,与食管良性肿瘤(13.3%,0)和癌旁食管正常黏膜组织(0,0)比较,差异均有显著性(P<0.001)。survivin和p53的表达强度与ESC的分化程度、临床分期和淋巴结转移均相关(P<0.05),而与肿瘤长度及部位均无相关(P>0.05)。survivin与p53的表达呈显著相关(P<0.01)。结论survivin和p53均与ESC的发生发展有关,二者在ESC的发展过程中可能起协同作用。联合检测survivin和p53在ESC中的表达水平,对判定其恶性程度及预后可能具有重要参考价值。

Objective To study the expression of survivin and p53 and their significance in esophageal squamous carcinoma（ESC）. Methods The expression of survivin and p53 was examined immunohistochemically by SP and PowerVision^TM-9000（PV-9000） methods in 60 cases of ESC, 15 cases of esophageal benign tumor and 10 specimens of adjacent normal esophageal mucosa.Results The positive expression rate of survivin and p53 in ESC were 71.67% and 65.00%, respectively. There was significant difference between ESC and esophaseal benign tumor（13.33 %, 0）, adjacent normal mucosa （0,0, P 〈 0.001）. The expression intensity of survivin and p53 correlated with histopathological grade, clinical stage and lymph node metastasis （ P 〈 0.05）. There was no correlation between the expression intensity of survivin, p53 and the location, the length of ESC （ P 〉 0.05）. Kendall positive correlation was found between the expressive intensity of survivin and p53 （ P 〈 0.01）. Conclusion The results suggest that survivin and p53 may play certain roles in the oncogenesis and progression of ESC, and may have synergetic action in the development of ESC. Ctimbined detection of the expression of survivin and p53 may be useful for evaluating malignancy and prognosis.