期刊文献+

人支气管上皮细胞恶变过程中生物学特性及超微结构的研究 被引量:1

Study on Biological Characteristic and Ultrastructure During the Malignant Transformation of Human Bronchial Epithelial Cell
下载PDF
导出
摘要 目的:探讨烟草特异性亚硝胺(NNK)诱发人支气管上皮细胞(BEAS-2B)恶性转化过程中生物学特性及超微结构变化情况。方法:以500μg/ml的NNK处理BEAS-2B细胞24小时后,细胞在体外连续传代培养,在此过程中观测细胞生物学特性及超微结构的变化情况。结果:第5代细胞显示抗血清生长,细胞在裸鼠体内不成瘤。与对照细胞相比,细胞及细胞核形态、细胞器形态及数量无显著变化;第15代细胞在半固体琼脂中克隆形成率(0.032%)为对照细胞(0.0023%)的13.9倍,细胞在裸鼠体内不成瘤。细胞超微结构显示转化细胞特征;第25代细胞在裸鼠体内成瘤,组织学证实为鳞癌(Ⅰ~Ⅱ级)。细胞超微结构显示具有明显的肿瘤细胞特征。结论:浓度为500μg/ml的NNK可成功诱发BEAS-2B细胞恶性转化,在此过程中,细胞超微结构及生物学特性逐步改变,提示人支气管上皮细胞恶性转化过程是一个多步骤、多阶段的发展过程。 Objective: To study the biological characteristic and ultrastructure during the malignant transformation of human bronchial epithelial cell(BEAS-2B) induced by tobacco-specific nitrosamine(NNK). Methods: BEAS-2B cells treated with NNK at the concentration of 500μg/ml for 24 hours were subcultured continuously in vitro and biological characteristic and ultrastructure of them were observed. Results: The serum resistance was significantly increased in the 5th passage BEAS-2BNNK cells and could not grow into tumor in nude mice. Compared with control ceils, there was no signicfiant difference in ultrastructrure; The plating efficiency of the 15th passage BEAS-2BNNK cells in soft agar(0. 032% ) increased 13.9-fold compared with that of control cells(0. 0023% ) The BEAS-2BNNK ceils bad the biological characteristic of transformation cells but could not grow into tumor in nude mice; The 25th passage ceils could grow into tumor in nude mice. The tumor was a squamous cell carcinoma in morphology confirmed by histopathological examination. The ultrastructrure showed that BEAS-2BNNK cells were Transformed into cancer cells. Condusion: The model of malignant transformation of BEAS-2B ceils induced by NNK(500μg/ml ) can be created successfully. During the period, the change of biological characteristic and ultrastructure indicated that the malignant transformation of human bronchial epithelial cell is a multiple-step course.
出处 《四川肿瘤防治》 2005年第4期209-212,共4页 Sichuan Journal of Cancer Control
关键词 人支气管上皮细胞 恶性转化 超微结构 BEAS-2B Nalignant Transformation Ultrastructure
  • 相关文献

参考文献9

  • 1Nishikawa A, Nori Y, Lee IS, et, 81. Cigarette smoking,metabolic activation and carcinogenesis [J]. Curr Erug Metab,2004,5 ( 5 ) :363 -373.
  • 2Willey JC. Immortlization of normal human bronchial epithlial cells by papiIIomviruses 16 or 18 [J]. Cancer Res, 1991,51 : 5370-5377.
  • 3Hie TK. Malignant transformation of human bronchial epithelial cells by radon-simulated α-partical [ J ]. Carcinogenesis, 1994,15(3) :431-437.
  • 4Suzuki K,Hei TK. Mutation induction in γ-irradiated primary human bronchial epithelial cells and molecular analysis of the HPRT-mutants [J]. Mut Res,1996,349:33-41.
  • 5Zhou H,Calaf GM, Hei TK. Malignant transformation of human bronchial epithelial cells with the tobacco-specific nitxusamine, 4-(methylnitrosamino) -1-butanone [ J ]. Int J Cancer , 2003, 106(6) :821-826.
  • 6Hei TK. Radon-induced neoplastic transformation of human bronchial epithelial cells [ J ]. Radi Oncol Inv. 1996,3:398-404.
  • 7孙红琰,龚诒芬,王全立,杜芝燕,杨陟华.烟草特异亚硝胺NNK诱发人胎肺细胞癌变启动模型的建立[J].中国公共卫生学报,1998,17(5):282-284. 被引量:3
  • 8朱茂祥,杨陟华,龚诒芬,徐勇.NNK诱发BEP2D细胞产生活性氧及其对DNA的损伤[J].中国生物化学与分子生物学报,1999,15(6):992-996. 被引量:7
  • 9Lunec J,Herbert K. 8-Hydroxy-deoxyguanosine,A marker of oxidative DNA damage in systemic lupus erthrocytes [ J ]. FEBS Letts,1994,348 : 131-133.

二级参考文献10

共引文献8

同被引文献4

引证文献1

二级引证文献2

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部