用PCR－RFLP方法研究新疆地区汉族HLA－DQA1和－DQB1基因多态性

HLA-DQA1 AND-DQB1 GENOTYPING BY PCR-RFLP IN XIN-JIANG HAN CHINESE

应用ＰＣＲ－ＲＦＬＰ技术，对新疆地区汉族健康群体进行了ＨＬＡ－ＤＱＡ１（４９人）和－ＤＱＢ１（４７人）基因分型。在ＤＱＡ１８个等位基因中，ＤＱＡ１０３０１的基因频率最高（３２．５６％），０４０１最低（１．０２％）。在ＤＱＢ１１６个等位基因中，ＤＱＢ１０２０１（２０．２１％）、０３０１（１５．９６％）、０３０３（１４．８９％）为最常见；没有观察到０５０３２、０５０４和０６０５。与河北固安县及江浙沪地区汉族群体进行比较，ＤＱＡ１基因未发现存在差异。而ＤＢ１０６０２（３．１９％）与固安汉族（Ｐｃ＝０．０１４４）和上海汉族（Ｐｃ＝０．０１４０）有显著差异，ＤＱＢ１０５０３（８．５２％）与上海汉族有显著差异（Ｐｃ＝０．０２１６）。

HLA-DQA1(49 individuals)and -DQB1(47 individuals)were investigated in a Han Chinese population of Xinjiang Autonomous Region, using PCR-RFLP genotyping method.Of the 8 DOA1 alleles detected,QA1 030l(gene frequency:32.56%)was the most common,and 0401(1.02%)was the rarest.No DQA1 difference was found between this population and Gu′an Hans and Jiangsu,Zhejiang,Shanghai Hans. Of the 16 DQB1 alleles tested,DQB1 05032,0504 and 0605 were absent.DQB1, 0201 (20. 21%),0301(15.96%)and 0303(14. 89%)were much more common than the other alleles, which is in accordance with those of Gu′an Hans and Shanghai Hans, There was a significant difference in DQB1 0602 between Xinjiang Hans(3. 19%)and Gu′an Hans(12.1%, Pc=0.0144)and Shanghai Hans(12. 36%, Pc=0. 0140) , and so was DQB1 0503 between Xinjiang Hans (8.52%)and Shanghai Hans(0. 56%, Pc=0. 0216)。