rIL－6抗癌机理初探（Ⅱ）：rIL－6对小鼠LAK细胞体内外调节效应

EFFECT OF RECOMBINANT HUMAN INTERLEUKIN-6 ONMURINE LAK CELL IN VITRO AND VIVO

为探讨ｒＩＬ－６对ＬＡＫ细胞的调节效应，进行了ｒＩＬ－６对小鼠脾脏ＬＡＫ细胞功能影响的体内外实验研究。实验采用Ｃ_（５７）ＢＬ／６荷瘤或正常小鼠，给予ｒＩＬ－６或生理盐水，１５ｄ后取小鼠脾细胞诱导活化后进行ＬＡＫ杀伤功能测定。结果表明①ｒＩＬ－６在体外对ＬＡＫ杀伤活性有明显的促进作用并在一定范围内呈剂量反应关系；②体内注射ｒＩＬ－６后对正常鼠和荷瘤鼠ＬＡＫ细胞的诱导及杀伤活性均有促进作用；③荷瘤对正常鼠ＬＡＫ细胞功能有抑制作用，而ｒＩＬ－６对荷瘤所致ＬＡＫ细胞功能下降有一定的预防作用。提示ｒＩＬ－６对小鼠脾脏ＬＡＫ细胞杀伤活性的促进效应可能是ｒＩＬ－６发挥抗癌作用的一个途径。

A study on the effect of rIL-6 on murine LAK in vitro and vivo was taken.The normal C57BL/6mice and the EL4 carring mice was injected with IL-6(10μg/day,i.P. tid) or NS respectively for 10 days and at 15th the spleen was treated to single cell suspension and LAK activity was induced by IL-2( 1 000U/ml) for 3 days. The result showed that①the LAK activity was improved by 50～200U/ml of IL-6. The optimal dose was 200U/ml, on which the LAK function increased from 35. 75%to 65; 50%;②IL-6 may improve the LAK function through promoting their cytotoxicity and proliferation within normal mice or tumor carring mice models;③although tumor carring decreased the LAK's function,the IL-6 could regulated the immunological function to antagonize the immunological inhibitions.