摘要
对我所建成的鼻咽低分化癌的EB病毒核抗原阳性(EBNA)的裸鼠移植瘤SUNT-1及其体外培养上皮细胞株SUNE-1,分别于各代进行细胞遗传学分析,结果显示SUNT-1和SUNE-1癌细胞染色体的数目和结构均有相似的变化;染色体数呈非整倍体改变,并以亚四倍体为主,染色体畸变则以易位、重排、缺乏和等臂染色体为多见并形成较恒定的标记染色体M1、M2、M3、M5和M6,涉及3号染色体改变较多。提示进一步研究克隆SUNF-1和SUNT-1亚株的细胞遗传学和EB病毒基因存在变化,将为深入探讨鼻咽癌病因提供更多启示。
The cytogenetics of the xenografted tumor in nude mouse(SUNT-1)and its correspondingepithelial cell line(SUNE-1) in the cell culture derived from a nasopharyngeal tumor of poorly differentiat-ed squamous carcinoma has been investigated.It was demonstrated that the chromosome number of theSUNT-1 and SUNE-1 cells varied from 52 to 84, but the chromosome numbers of more than 70 % cellswere hypotetraploid.All cell of SUNT-1 and SUNE-1 contained many abnormal chromosonlal changes(translocation,deletion, rearrangement,isochromosome).There were a series of characteristic marker chro-mosomes.Most of them were either consistent or found repeatedly,such as M1,M2,M3,M5 and M6.Theresults suggested that further study on the cytogenetic and EBV genes of the SUNT-1 and SUNE-1 sub-clone would be helpful for seeking NPC etoliogy.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1996年第2期84-86,共3页
Chinese Journal of Cancer
基金
美国中华医学基金
关键词
鼻咽肿瘤
移植瘤
上皮细胞株
细胞遗传学
Nasopharyngeal Nude mouse transplanted tumor Epithelial cell line ChromosomeEBNA