摘要
The effect of lanthanum ( Ⅲ ) (La^3 + ) on cytosolic free calcium ( [ Ca^2 + ] i ) in isolated rabbit mature osteoclasts was studied with the employment of fluo-3/AM as an intracellular calcium-sensitive fluorescent probe by using a confocal laser scanning microscope. La^3+ does not alter basal [Ca^2+ ]i levels and cell spread area at the concentration of 1.00 × 10^- 8 mol· L ^- 1. However, La^3 + at higher concentrations ( 1. 00 × 10^ - 5 and 1.00 × 10^- 7 mol· L^- 1 ) decreases [ Ca^2 + ] i levels and cell spread area, and greater decreases are observed for the higher concentrations of La^3 + . Since [Ca^2 + ]i affects cytoskeleton and the adhesion properties of osteoclasts, our results seem to suggest that La^3 + inhibit bone resorption by decreasing [Ca^2+]i in rabbit mature osteoclasts.
The effect of lanthanum ( Ⅲ ) (La^3 + ) on cytosolic free calcium ( [ Ca^2 + ] i ) in isolated rabbit mature osteoclasts was studied with the employment of fluo-3/AM as an intracellular calcium-sensitive fluorescent probe by using a confocal laser scanning microscope. La^3+ does not alter basal [Ca^2+ ]i levels and cell spread area at the concentration of 1.00 × 10^- 8 mol· L ^- 1. However, La^3 + at higher concentrations ( 1. 00 × 10^ - 5 and 1.00 × 10^- 7 mol· L^- 1 ) decreases [ Ca^2 + ] i levels and cell spread area, and greater decreases are observed for the higher concentrations of La^3 + . Since [Ca^2 + ]i affects cytoskeleton and the adhesion properties of osteoclasts, our results seem to suggest that La^3 + inhibit bone resorption by decreasing [Ca^2+]i in rabbit mature osteoclasts.
基金
ProjectsupportedbytheNationalNaturalScienceFoundationofChina(20031010,20271005)