尼美舒利致突变的微核试验研究

Study on Mutagenicity of Nimesulide Induced Micronucleus Frequency in Mouse Bone Marrow Polychromatophilic Erythrocytes

细胞中微核的出现能反映出染色体畸变的情况,微核率与染色体畸变率之间的相关性显著,因而已将微核率作为测定细胞损伤程度的一种敏感指标。本文运用小鼠骨髓细胞微核试验方法对新型抗炎药尼美舒利进行遗传毒理学测试。以37.5,70,150,300 mg/kg 体重剂量作一次性用药剂量,以37.5 mg/kg 体重剂量间隔24 h 分3 d 连续用药。结果显示,不同剂量及不同给药时间所致微核率为1.9‰～2.3‰,与阴性对照组的差异无显著性(P>0.05)。由此表明,尼美舒利在动物体内无明显致突变性。

It was reported in our earlier work that nimesulde may be not a mutagenis agent in the Ames test.In this paper,the mieronucleus frequency in mouse bone marrow polychromatophilic ery- throcytes was used as a criterion to further explore the mutagenicity of the drug in vivo.35 male and 35 female mice of the K unming strain (average body wt:21.2±3.7 g) were divided randomly into seven groups according to their weight.Four of these groups were treated with the concen- tration of the nimesulide 37.5,75,150 and 300 mg/kg one time.A group was treated three times at 0.24 and 48 hours with the concentration of 37.5 mg/kg.The results showed that the mi- cronucleus frequency of the drug ranged from 1.9‰～2.3‰.There was no significantly differ- ence between the treated groups and the negative control group.It suggests that nimesulide may not be a mutagenis agent in vivo.