基因扩增法检测肺炎衣原体DNA的研究

Detection of Chlamydia Pneumoniae by Polymerase Chain Reaction

采用聚合酶链式反应（PCR），建立检测肺炎衣原体DNA的实验诊断方法。结果显示：采用肺炎衣原体PstI编码区的两对特异性引物（HL－1-HR－1和HM－1-HR-1）进行扩增，两株肺炎在原体（TW－183和CM－1）均可见437bP和229bP的特异性扩增带，而沙眼衣裳原体和鹦鹉热在原体扩增结果为阴性。检测敏感性为100fg左右。该技术为肺炎衣原体的早期诊断和流行病学调查提供了一种快速、敏感、特异的新方法。

?We have developed the method for detection of Chlamydia pneumoniae, based upon the polymerase chain reaction (PCR). A cloned C. Pneumoniae 474bp Pst I fragment was sequenced and two specific restriction fragnients were chosen asprimers for use in PCR. The results showed that two strains of C. pneumoniae (TW-183 and CM-1)tested were amplified by the HL-1-HR-1primer pair or the HM-1-HR-1 primer pair, producing the expected 437bp and 229bp amplification products, respec-tively. Nevertheless upon serotyping,no chlamydia trachomatis serovars,chlamydia psittaci strains, or other organisme tested wereamplified. Bands of positive amplified DNA were detected in 100fg template DNA. Our study suggested that this technique couldbe applied to clinical specimens for rapid identification and specific diagnosis of chlamydia pneumoniae.