糖尿病肾病患者血清CC趋化因子升高并与AGE-肽相关

Elevated CC chemokines correlate with AGE-peptide in the serum of patients with diabetic nephropathy

目的:观察糖尿病肾病(DN)患者血清单核细胞趋化蛋白-1(MCP-1)和受活化调节、由正常T细胞表达分泌的趋化蛋白(RANTES)水平,并探讨它们与糖基化终产物-肽(AGE-P)的相互关系。方法:设健康对照(NC)组46例,糖尿病(DM)患者176例分为正常蛋白尿(DM N)组、微量白蛋白尿(DM Mi)组及显性蛋白尿(DM Ma)组。流动注射分析法检测血清AGE-P,ELISA法检测血清MCP-1和RANTES。结果:(1)DM患者血清MCP-1、RANTES和AGE-P水平均明显高于NC组(均为P<0.01);(2)DM Ma组和DM Mi组血清MCP-1水平均显著高于DM N组(均为P<0.05);(3)DM Ma组血清AGE-P水平明显高于DM Mi组和DM N组(P<0.05和P<0.01);(4)DN患者血清AGE-P与RANTES及MCP-1水平之间呈正相关(r=0.31,P<0.05;r=0.53,P<0.001)。结论:DM患者血清AGE-P、RANTES和MCP-1水平升高;血清AGE-P及MCP-1水平与DN病变程度平行,DN患者血清AGE-P与RANTES及MCP-1呈正相关。

Objective To investigate the serum level of MCP-1 and RANTES in patients with diabetic nephropathy （DN） and explore the relationship between advanced glycosylation end products-peptide （AGE-P） and these two chemokines. Methods 46 healthy people and 176 diabetic patients （DM） were included in the study. Serum AGE-P was measured by using flow injection assay. MCP-1 and RANTES was determined by using ELISA. Results The serum MCP-1, RANTES and AGE-P were significantly increased compared to normal control （ P 〈 0.01 ）. The serum MCP-1 instead of RANTES in patients with overt proteinufia and micro-albuminuria were significant higher than that in DM patients without albuminuria （ P 〈 0.05）. Serum AGE-P in patients with overt proteinufia was significant higher than that in DM patients with micro-albuminuria （ P 〈 0.05） or without albuminuria （P 〈 0.01）. Serum AGE-P showed significantly positive correlation with MCP-1 （r = 0.53, P 〈 0.001 ） and RANTES （ r = 0.31, P 〈 0.05） in DN patients. Conclusions Serum AGE-P, MCP- 1 and RANTES are increased in DM patients. Serum AGE-P correlates to MCP-1 and RANTES. These data suggest that the interaction of AGEs with CC chemokines may contribute to the development of DN.